Stazione Sperimentale per l'Industria delle Conserve Alimentari, V.le F. Tanara, 31/A, Parma 43121, Italy.
Toxins (Basel). 2012 Feb;4(2):68-82. doi: 10.3390/toxins4020068. Epub 2012 Feb 1.
Twelve yeast strains isolated from the surface of Italian typical dry-cured hams, belonging to D. hansenii, D. maramus, C. famata, C. zeylanoides and H. burtonii species, and previously selected for their ability to grow in dry-cured ham-like substrates, were screened for antagonistic activity against a toxigenic strain of P. nordicum and inhibition of ochratoxin A (OTA) biosynthesis. On average, yeast inhibitory activity was lowered by increasing fungal inoculum and enhanced by NaCl presence. In the assay conditions, H. burtonii and C. zeylanoides were the most effective, both in inhibiting P. nordicum growth and OTA production. D. hansenii was the species with the lowest inhibitory activity, especially in the absence of salt. OTA production dropped from the range < LOD - 5000 ppb in P. nordicum control plates to the range < LOD - 200 ppb in yeast-added plates. OTA production increased in the presence of NaCl in P. nordicum control plates, while salt enhanced inhibition against OTA production in yeast-added plates.
从意大利典型干腌火腿表面分离出的 12 株酵母菌株，属于 Hansenula hansenii、Debaryomyces maramus、Candida famata、Candida zeylanoides 和 Hanseniaspora burtonii 种，先前因其在干腌火腿样基质中生长的能力而被选中，对产毒菌株的拮抗活性进行了筛选。青霉和抑制赭曲霉毒素 A（OTA）生物合成。平均而言，随着真菌接种物的增加，酵母抑制活性降低，而 NaCl 的存在增强了抑制活性。在试验条件下，汉逊酵母和热带假丝酵母最有效，既能抑制青霉生长，又能抑制 OTA 产生。汉森酵母的抑制活性最低，特别是在没有盐的情况下。OTA 的产量从青霉对照平板中的<LOD-5000 ppb 范围降至添加酵母平板中的<LOD-200 ppb 范围。在青霉对照平板中添加 NaCl 会增加 OTA 的产量，而盐会增强添加酵母平板中对 OTA 产量的抑制作用。
