Iacumin Lucilla, Arnoldi Martina, Comi Giuseppe
Department of Agricultural, Food, Environmental and Animal Science, University of Udine, Via Sondrio 2/a, 33100 Udine, Italy.
Microorganisms. 2020 Oct 21;8(10):1623. doi: 10.3390/microorganisms8101623.
Recently, specific dry-cured hams have started to be produced in San Daniele and Parma areas. The ingredients are similar to protected denomination of origin (PDO) produced in San Daniele or Parma areas, and include pork leg, coming from pigs bred in the Italian peninsula, salt and spices. However, these specific new products cannot be marked as a PDO, either San Daniele or Parma dry cured ham, because they are seasoned for 6 months, and the mark PDO is given only to products seasoned over 13 months. Consequently, these products are called short-seasoned dry-cured ham (SSDCH) and are not branded PDO. During their seasoning period, particularly from the first drying until the end of the seasoning period, many molds, including spp. and spp., can grow on the surface and work together with other molds and tissue enzymes to produce a unique aroma. Both of these strains typically predominate over other molds. However, molds producing ochratoxins, such as and , can simultaneously grow and produce ochratoxin A (OTA). Consequently, these dry-cured hams may represent a potential health risk for consumers. Recently, has been isolated from SSDCHs, which could represent a potential problem for consumers. Therefore, the aim of this study was to inhibit using or or a mix of both microorganisms. Six and six strains were tested in vitro for their ability to inhibit . The strains (DIAL)1 and (Lb)4 demonstrated the highest inhibitory activity and were selected for in situ tests. The strains were inoculated or co-inoculated on fresh pork legs for SSDCH production with OTA-producing prior to the first drying and seasoning. At the end of seasoning (six months), OTA was not detected in the SSDCH treated with both microorganisms and their combination. Because both strains did not adversely affect the SSDCH odor or flavor, the combination of these strains are proposed for use as starters to inhibit OTA-producing .
最近,圣丹尼尔和帕尔马地区开始生产特定的干腌火腿。其原料与圣丹尼尔或帕尔马地区生产的受保护原产地名称(PDO)火腿相似,包括来自意大利半岛养殖猪的猪腿、盐和香料。然而,这些特定的新产品不能标注为PDO,既不能是圣丹尼尔干腌火腿也不能是帕尔马干腌火腿,因为它们的腌制时间为6个月,而PDO标志只授予腌制时间超过13个月的产品。因此,这些产品被称为短期腌制干腌火腿(SSDCH),且没有PDO品牌。在腌制期间,特别是从初次干燥到腌制期结束,许多霉菌,包括 spp. 和 spp.,会在表面生长,并与其他霉菌和组织酶共同作用产生独特的香气。这两种菌株通常比其他霉菌占优势。然而,产生赭曲霉毒素的霉菌,如 和 ,可能同时生长并产生赭曲霉毒素A(OTA)。因此,这些干腌火腿可能对消费者构成潜在的健康风险。最近,已从SSDCH中分离出 ,这可能对消费者构成潜在问题。因此,本研究的目的是使用 或 或两者的混合物来抑制 。对6株 和6株 菌株进行了体外抑制 的能力测试。菌株 (DIAL)1和 (Lb)4表现出最高的抑制活性,并被选用于原位测试。在初次干燥和腌制之前,将这些菌株接种或共同接种到用于生产SSDCH的新鲜猪腿上,并接种产生OTA的 。在腌制结束时(6个月),用两种微生物及其组合处理的SSDCH中未检测到OTA。由于这两种菌株均未对SSDCH的气味或风味产生不利影响,因此建议将这些菌株的组合用作起始剂以抑制产生OTA的 。