Cárdenas José, Rojas Janne, Rojas-Fermin Luís, Lucena María, Buitrago Alexis
Organic Biomolecular Research Group, Research Institute, Faculty of Pharmacy and Bioanalysis, University of Los Andes, Mérida, Venezuela.
Nat Prod Commun. 2012 Feb;7(2):243-4.
The essential oils from fresh aerial parts of Monticalia greenmaniana (Hieron) C. Jeffrey (Asteraceae) collected in March, were analyzed by GC/MS. Oil yields (w/v) of 0.1% (flowers), 0.07%, (stems) and 0.1% (leaves) were obtained by hydrodistillation. Thirteen, sixteen and eighteen components, respectively, were identified by comparison of their mass spectra with those in the Wiley GC-MS Library data base. The major components of the flower and stem oils were 1-nonane (38.8% flowers; 33.5% stems), alpha-pinene (29.0% flowers; 14.8% stems) and germacrene D (15.6% flowers; 18.6% stems). However, in the leaf oil, germacrene D was observed at 50.7%, followed by beta-cedrene at 8.4%. The leaf essential oil showed a broad spectrum of antibacterial activity against the important human pathogenic Gram-positive and Gram-negative bacteria Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATCC 19433), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and Klebsiella pneumoniae (ATCC 25955) with MIC values ranging from 75 to 6000 ppm.
对3月采集的绿山蒙特卡利亚菊（Monticalia greenmaniana (Hieron) C. Jeffrey，菊科）新鲜地上部分的精油进行了气相色谱/质谱分析。通过水蒸馏法获得的出油率（重量/体积）分别为：花0.1%、茎0.07%、叶0.1%。通过将其质谱与Wiley气相色谱-质谱数据库中的质谱进行比较，分别鉴定出了13种、16种和18种成分。花油和茎油的主要成分是壬烷（花中占38.8%；茎中占33.5%）、α-蒎烯（花中占29.0%；茎中占14.8%）和杜松烯D（花中占15.6%；茎中占18.6%）。然而，叶油中杜松烯D的含量为50.7%，其次是β-雪松烯，含量为8.4%。叶精油对重要的人类致病革兰氏阳性菌和革兰氏阴性菌金黄色葡萄球菌（ATCC 25923）、粪肠球菌（ATCC 19433）、大肠杆菌（ATCC 25922）、铜绿假单胞菌（ATCC 27853）和肺炎克雷伯菌（ATCC 25955）表现出广谱抗菌活性，最低抑菌浓度值在75至6000 ppm之间。
