Kuai Yan-Rong, He Zhan-Ju, Cai Xue-Yong, Wan Sheng, Yuan Yi-Ming, Peng Jing, Zhang Lei, Xue Qing, Shang Jing, Chen Fei, Ju Hui-Yan, Zuo Wen-Li, Liao Qin-Ping, Xu Yang
Department of Gynecology and Obstetrics, Peking University First Hospital, Beijing 100034, China.
Zhonghua Nan Ke Xue. 2012 Mar;18(3):231-4.
To investigate the effectiveness of testicular sperm cryopreservation in male fertility preservation by evaluating the clinical outcome of ICSI cycles with frozen-thawed testicular sperm for azoospermia patients.
We retrospectively analyzed 96 samples of cryopreserved testicular sperm obtained by testicular biopsy, vasovasostomy (V-V), vasoepididymostomy (V-E) , of which 55 were subjected to 60 ICSI cycles with frozen-thawed testicular sperm. We evaluated the rates of sperm recovery, fertilization, cleavage, transferable and good-quality embryos, clinical pregnancy, pregnancy outcome, and health of the newborns.
All the frozen testicular sperm samples were recovered successfully. The rates of fertilization, 2PN fertilization, cleavage, available embryos and good-quality embryos were 77.6, 69.4, 99.4, 84.5 and 40.8%, respectively. There were transferable embryos in all cycles. Fresh embryos were transferred in 52 of the 60 cycles, with the clinical pregnancy rate of 57.7% (30/52), including 19 singletons and 11 twins, and the rates of implantation and miscarriage were 38.7% (41/106) and 3.33% (1/30). Up to the present time, there have been 20 healthy newborns, including 12 boys and 8 girls, and another 13 ongoing pregnancies. No birth defects have been found so far.
Desirable clinical outcomes can be obtained from ICSI cycles with frozen-thawed testicular sperm, and testicular sperm cryopreservation is an effective method of fertility preservation for azoospermia males.
通过评估无精子症患者冻融睾丸精子的卵胞浆内单精子注射（ICSI）周期的临床结局，探讨睾丸精子冷冻保存对男性生育力保存的有效性。
我们回顾性分析了通过睾丸活检、输精管吻合术（V-V）、附睾输精管吻合术（V-E）获得的96份冷冻保存的睾丸精子样本，其中55份进行了60个冻融睾丸精子的ICSI周期。我们评估了精子复苏率、受精率、卵裂率、可移植胚胎率、优质胚胎率、临床妊娠率、妊娠结局和新生儿健康状况。
所有冷冻的睾丸精子样本均成功复苏。受精率、2PN受精率、卵裂率、可用胚胎率和优质胚胎率分别为77.6%、69.4%、99.4%、84.5%和40.8%。所有周期均有可移植胚胎。60个周期中有52个周期移植了新鲜胚胎，临床妊娠率为57.7%（30/52），包括19例单胎和11例双胎，着床率和流产率分别为38.7%（41/106）和3.33%（1/30）。截至目前，已有20名健康新生儿，包括12名男孩和8名女孩，另有13例正在妊娠中。目前尚未发现出生缺陷。
冻融睾丸精子的ICSI周期可获得理想的临床结局，睾丸精子冷冻保存是无精子症男性生育力保存的有效方法。
