Department of Orthopaedics, The Second Hospital of Shanxi Medical University, 382 Wuyi Road, Taiyuan, 030001, Shanxi, People's Republic of China.
Knee Surg Sports Traumatol Arthrosc. 2013 Aug;21(8):1751-62. doi: 10.1007/s00167-012-1977-7. Epub 2012 Apr 4.
To determine the importance of synovial fluid (SF) or subchondral bone marrow (BM) as nutrition sources in cartilage degeneration.
Ninety-five-month-old male rabbits were randomly divided into 5 groups according to sources of nutrition: SFBM-both; BM-only; SF-only; None-SFBM; and Free plug (unrestricted). Nutrition to 4-mm-diameter cylindrical osteochondral plugs created on the trochlea of the distal femurs was obstructed by Polyvinyl Chloride (PVC) cap. Cartilage changes were assessed after 4, 8, and 12 weeks by histology, immunohistochemistry, and real-time PCR.
Cartilage in the BM-only group suffered the greatest damage, followed by the None-SFBM and SF-only groups. Apoptosis was increased in the BM-only and None-SFBM groups compared with others. Cartilage was significantly thinner at all time points in the BM-only and None-SFBM groups when compared with SFBM-both and Free plug, whereas in the SF-only group, this difference occurred after 8 weeks. Compared with SFBM-both and Free plug, expression of collagen II and aggrecan mRNAs in all groups was decreased but MMP-3 increased, respectively.
Our data indicate that SF-derived nutrition is the dominant source of sustenance for adult cartilage structure and function. Cartilage damage is observed when the only nutrition source is the BM.
确定滑液（SF）或软骨下骨髓（BM）作为软骨退变营养来源的重要性。
95 月龄雄性兔根据营养来源随机分为 5 组：SFBM-两者；BM-仅；SF-仅；SFBM-无；和自由塞（无限制）。通过聚氯乙烯（PVC）帽阻断在股骨远端滑车处创建的 4mm 直径的骨软骨圆柱营养。通过组织学、免疫组织化学和实时 PCR 在 4、8 和 12 周后评估软骨变化。
BM-仅组的软骨损伤最大，其次是 SFBM-无和 SF-仅组。与其他组相比，BM-仅和 SFBM-无组的细胞凋亡增加。与 SFBM-两者和自由塞相比，BM-仅和 SFBM-无组的软骨在所有时间点均明显变薄，而在 SF-仅组中，这种差异发生在 8 周后。与 SFBM-两者和自由塞相比，所有组的胶原 II 和聚集蛋白聚糖 mRNA 的表达均降低，但 MMP-3 分别增加。
我们的数据表明 SF 来源的营养是维持成年软骨结构和功能的主要营养来源。当唯一的营养来源是 BM 时，会观察到软骨损伤。
