Institute of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.
J Thromb Thrombolysis. 2012 Aug;34(2):260-8. doi: 10.1007/s11239-012-0701-2.
Toll-like receptors (TLRs) have been widely known for their role as an important mediator of immune reactions in the development and progression of atherosclerotic (AS) lesions, which play the most important role in the occurrence of cerebral infarction (CI). The lipopolysaccharide (LPS)-lipopolysaccharide binding protein (LBP) compounds connect LPS receptor-CD14 and play an important role in promoting inflammatory reaction through TLRs. This study was designed to study whether LBP genetic polymorphisms are associated with CI risk in a Chinese population. LBP c.291T>C and c.1306T>C single nucleotide polymorphisms (SNPs) were genotyped in 366 CI cases and 200 controls from Changsha by using method of Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Blood lipid and lipoprotein levels were also detected and the intima-media thickness (IMT) of carotid arteries were determined. No significant difference in genotype and allele distribution of the LBP c.291T>C and LBP c.1306T>C polymorphism was observed between the cases and controls (P > 0.05). CI patients carrying the TC genotype for both LBP c.291T>C and c.1306T>C polymorphisms showed higher carotid IMT than those carrying the TT genotype (P < 0.05, respectively). LBP c.291T>C and c.1306T>C polymorphisms were significantly associated with carotid IMT in Changsha, China, but both polymorphisms were not associated with risk of atherosclerotic CI.
Toll 样受体（TLRs）作为动脉粥样硬化（AS）病变中免疫反应的重要介质而广为人知，在脑梗死（CI）的发生中起着最重要的作用。脂多糖（LPS）-脂多糖结合蛋白（LBP）复合物连接 LPS 受体-CD14，并通过 TLRs 发挥重要作用促进炎症反应。本研究旨在研究中国人群中 LBP 遗传多态性与 CI 风险的关系。采用聚合酶链反应限制片段长度多态性（PCR-RFLP）方法，对 366 例 CI 病例和 200 例来自长沙的对照者的 LBP c.291T>C 和 c.1306T>C 单核苷酸多态性（SNP）进行基因分型。还检测了血脂和脂蛋白水平，并确定了颈动脉内膜中层厚度（IMT）。LBP c.291T>C 和 LBP c.1306T>C 多态性的基因型和等位基因分布在病例和对照组之间无显著差异（P > 0.05）。携带 LBP c.291T>C 和 c.1306T>C 多态性 TC 基因型的 CI 患者的颈动脉 IMT 高于携带 TT 基因型的患者（分别为 P < 0.05）。LBP c.291T>C 和 c.1306T>C 多态性与中国长沙的颈动脉 IMT 显著相关，但两种多态性均与动脉粥样硬化性 CI 风险无关。
