LEPAE, Departamento de Engenharia Química, Faculdade de Engenharia da Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
Anal Bioanal Chem. 2012 Jun;403(6):1761-9. doi: 10.1007/s00216-012-5962-2. Epub 2012 Apr 5.
A methodology for the extraction and quantification of 16 polycyclic aromatic hydrocarbons (PAHs) based on microwave-assisted extraction coupled with headspace solid-phase microextraction followed by gas chromatography/mass spectroscopy was validated for needles and bark of two pine species (Pinus pinaster Ait. and Pinus pinea L.). The limits of detection were below 0.92 ng g(-1) (dry weight) for needles and below 0.43 ng g(-1) (dw) for bark. Recovery assays were performed with two sample masses spiked at three levels and the overall mean values were between 70 and 110 % for P. pinaster and 75 and 129 % for P. pinea. In the first species, the increase in sample mass lowered the recoveries slightly for most PAHs, whereas for the second, the recoveries were higher for the needles. Naturally contaminated samples from 4 sites were analysed, with higher levels for urban sites (1,320 and 942 ng g(-1) (dw) vs. 272 and 111 ng g(-1) (dw) for needles and 696 and 488 ng g(-1) (dw) vs. 270 and 103 ng g(-1) (dw) for bark) than for rural ones and also for P. pinaster samples over P. pinea. It is also shown that gas-phase PAHs are predominant in the needles (over 65 % of the total PAHs) and that the incidence for particulate material in bark, reaching 40 % as opposed to a maximum below 20 % for the needles. The method has proved to be fit and improved some of the existing approaches, on the assessment of particulate PAHs and bark levels.
基于微波辅助提取结合顶空固相微萃取与气相色谱/质谱联用的方法，对两种松树（PinuspineaL.和 PinuspineaL.）的针叶和树皮中 16 种多环芳烃（PAHs）进行了提取和定量方法的验证。该方法的检测限低于 0.92ngg(-1)（干重）的针状叶片和低于 0.43ngg(-1)（dw）的树皮。采用两种样品质量进行了加标回收率测定，加标水平为 3 个，整体平均值在 PinuspineaL.和 PinuspineaL.之间分别为 70%和 110%和 75%和 129%。在第一种物种中，随着样品质量的增加，大多数 PAHs 的回收率略有降低，而对于第二种，针叶的回收率更高。对来自 4 个地点的天然污染样品进行了分析，城市地区的含量较高（1320 和 942ngg(-1)（dw）与 272 和 111ngg(-1)（dw）的针叶和 696 和 488ngg(-1)（dw）与 270 和 103ngg(-1)（dw）的树皮）比农村地区和 PinuspineaL.的样品更高。也表明气相 PAHs 在针叶中占主导地位（占总 PAHs 的 65%以上），而在树皮中颗粒物质的发生率达到 40%，而不是最高低于 20%的针叶。该方法在评估颗粒状 PAHs 和树皮水平方面被证明是合适的，并改进了一些现有的方法。
