氢交换质谱法：我们是否已经脱离困境？
Hydrogen exchange mass spectrometry: are we out of the quicksand?
机构信息
Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115-5000, USA.
出版信息
J Am Soc Mass Spectrom. 2012 Jun;23(6):1003-10. doi: 10.1007/s13361-012-0377-z. Epub 2012 Apr 3.
Abstract
Although the use of hydrogen exchange (HX) mass spectrometry (MS) to study proteins and protein conformation is now over 20 years old, the perception lingers that it still has "issues." Is this method, in fact, still in the quicksand with many remaining obstacles to overcome? We do not think so. This critical insight addresses the "issues" and explores several broad questions including, have the limitations of HX MS been surmounted and has HX MS achieved "indispensable" status in the pantheon of protein structural analysis tools.
摘要
尽管利用氢交换（HX）质谱（MS）来研究蛋白质和蛋白质构象已经有 20 多年的历史了，但人们仍然认为它仍然存在“问题”。这种方法实际上是否仍处于困境之中，仍有许多障碍需要克服？我们不这么认为。本文批判性地探讨了“问题”，并探讨了几个广泛的问题，包括 HX MS 的局限性是否已经克服，以及 HX MS 是否在蛋白质结构分析工具的万神殿中达到了“不可或缺”的地位。
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