通过氢交换质谱法研究溶液相蛋白质的结构与动力学
Investigating solution-phase protein structure and dynamics by hydrogen exchange mass spectrometry.
作者信息
Morgan Christopher R, Engen John R
机构信息
Northeastern University, Boston, Massachusetts.
出版信息
Curr Protoc Protein Sci. 2009 Nov;Chapter 17:17.6.1-17.6.17. doi: 10.1002/0471140864.ps1706s58.
Abstract
By taking advantage of labeling methods such as hydrogen exchange (HX), many details about protein conformation, dynamics, and interactions can be revealed by mass spectrometry. In this unit, hydrogen exchange theory is discussed as it applies to HX-MS protocols, the practice of HX-MS including data analysis and interpretation is explained in detail, and recent advancements in technology which greatly increase the depth of information gained from the technique are highlighted.
摘要
通过利用诸如氢交换（HX）等标记方法，质谱可以揭示许多关于蛋白质构象、动力学和相互作用的细节。在本单元中，将讨论适用于HX-MS方案的氢交换理论，详细解释HX-MS的实践，包括数据分析和解释，并重点介绍技术上的最新进展，这些进展极大地增加了从该技术中获得的信息深度。
相似文献
1
Investigating solution-phase protein structure and dynamics by hydrogen exchange mass spectrometry.通过氢交换质谱法研究溶液相蛋白质的结构与动力学
Curr Protoc Protein Sci. 2009 Nov;Chapter 17:17.6.1-17.6.17. doi: 10.1002/0471140864.ps1706s58.
2
Probing protein structure and dynamics by hydrogen exchange-mass spectrometry.通过氢交换质谱法探究蛋白质结构与动力学
Curr Protoc Protein Sci. 2002 Aug;Chapter 17:17.6.1-17.6.18. doi: 10.1002/0471140864.ps1706s28.
3
H/D exchange and mass spectrometry in the studies of protein conformation and dynamics: is there a need for a top-down approach?蛋白质构象与动力学研究中的氢/氘交换和质谱分析：是否需要自上而下的方法？
Anal Chem. 2009 Oct 1;81(19):7892-9. doi: 10.1021/ac901366n.
4
Analysis of protein conformation and dynamics by hydrogen/deuterium exchange MS.通过氢/氘交换质谱分析蛋白质构象和动力学
Anal Chem. 2009 Oct 1;81(19):7870-5. doi: 10.1021/ac901154s.
5
Hydrogen-exchange mass spectrometry for the study of intrinsic disorder in proteins.用于研究蛋白质内在无序性的氢交换质谱法。
Biochim Biophys Acta. 2013 Jun;1834(6):1202-9. doi: 10.1016/j.bbapap.2012.10.009. Epub 2012 Oct 22.
6
Reference Parameters for Protein Hydrogen Exchange Rates.蛋白质氢交换速率的参考参数。
J Am Soc Mass Spectrom. 2018 Sep;29(9):1936-1939. doi: 10.1007/s13361-018-2021-z. Epub 2018 Jul 18.
7
Hydrogen Exchange Mass Spectrometry.氢交换质谱法
Methods Enzymol. 2016;566:335-56. doi: 10.1016/bs.mie.2015.06.035. Epub 2015 Jul 27.
8
Protein dynamics and conformational changes explored by hydrogen/deuterium exchange mass spectrometry.通过氢/氘交换质谱法探索蛋白质的动态和构象变化。
Curr Opin Struct Biol. 2019 Oct;58:305-313. doi: 10.1016/j.sbi.2019.06.007. Epub 2019 Jul 25.
9
Analyzing protein dynamics using hydrogen exchange mass spectrometry.利用氢交换质谱法分析蛋白质动力学。
J Vis Exp. 2013 Nov 29(81):50839. doi: 10.3791/50839.
10
Hydrogen exchange mass spectrometry: are we out of the quicksand?氢交换质谱法：我们是否已经脱离困境？
J Am Soc Mass Spectrom. 2012 Jun;23(6):1003-10. doi: 10.1007/s13361-012-0377-z. Epub 2012 Apr 3.
引用本文的文献
1
Domain coupling in activation of a family C GPCR.C类G蛋白偶联受体激活过程中的结构域偶联
Nat Chem Biol. 2025 Apr 25. doi: 10.1038/s41589-025-01895-3.
2
Exploring the CNOT1(800-999) HEAT Domain and Its Interactions with Tristetraprolin (TTP) as Revealed by Hydrogen/Deuterium Exchange Mass Spectrometry.通过氢/氘交换质谱法揭示的CNOT1(800 - 999) HEAT结构域及其与锌指蛋白（TTP）的相互作用
Biomolecules. 2025 Mar 11;15(3):403. doi: 10.3390/biom15030403.
3
GPR180 is a new member of the Golgi-dynamics domain seven-transmembrane helix protein family.GPR180 是高尔基体动力学域七跨膜螺旋蛋白家族的新成员。
Commun Biol. 2024 Nov 28;7(1):1588. doi: 10.1038/s42003-024-07260-9.
4
Domain coupling in activation of a family C GPCR.C类G蛋白偶联受体激活中的结构域偶联
bioRxiv. 2024 Feb 29:2024.02.28.582567. doi: 10.1101/2024.02.28.582567.
5
Next-generation retinoid X receptor agonists increase ATRA signaling in organotypic epithelium cultures and have distinct effects on receptor dynamics.下一代视黄酸 X 受体激动剂增加器官型上皮培养物中的 ATRA 信号，并对受体动力学产生不同的影响。
J Biol Chem. 2023 Jan;299(1):102746. doi: 10.1016/j.jbc.2022.102746. Epub 2022 Nov 24.
6
Using hydrogen-deuterium exchange mass spectrometry to characterize Mtr4 interactions with RNA.利用氢氘交换质谱技术来鉴定 Mtr4 与 RNA 的相互作用。
Methods Enzymol. 2022;673:475-516. doi: 10.1016/bs.mie.2022.04.002. Epub 2022 May 14.
7
Advances in Hydrogen/Deuterium Exchange Mass Spectrometry and the Pursuit of Challenging Biological Systems.氢/氘交换质谱技术的进展及对挑战性生物系统的探索。
Chem Rev. 2022 Apr 27;122(8):7562-7623. doi: 10.1021/acs.chemrev.1c00279. Epub 2021 Sep 7.
8
Identification of a Structural Determinant for Selective Targeting of HDMX.HDMX选择性靶向的结构决定因素的鉴定。
Structure. 2020 Jul 7;28(7):847-857.e5. doi: 10.1016/j.str.2020.04.011. Epub 2020 Apr 30.
9
D' domain region Arg782-Cys799 of von Willebrand factor contributes to factor VIII binding.血管性血友病因子 D' 结构域 Arg782-Cys799 有助于与因子 VIII 结合。
Haematologica. 2020 Jun;105(6):1695-1703. doi: 10.3324/haematol.2019.221994. Epub 2019 Sep 26.
10
Conformational dynamics of the human serotonin transporter during substrate and drug binding.人血清素转运体在底物和药物结合过程中的构象动力学。
Nat Commun. 2019 Apr 11;10(1):1687. doi: 10.1038/s41467-019-09675-z.
Zotero 插件