急性脊髓损伤诱导大鼠多个器官的遗传损伤。
Acute spinal cord injury induces genetic damage in multiple organs of rats.
机构信息
Departamento de Biociências, Universidade Federal de São Paulo, UNIFESP, Santos, SP, Brazil.
出版信息
Cell Mol Neurobiol. 2012 Aug;32(6):949-52. doi: 10.1007/s10571-012-9825-3. Epub 2012 Apr 3.
Abstract
Spinal cord injury (SCI) is a devastating condition with important functional and psychological consequences. However, the underlying mechanisms by which these alterations occur are still not fully understood. The aim of this study was to analyze genomic instability in multiple organs in the acute phase of SCI by means of single cell gel (comet) assay. Rats were randomly distributed into two groups (n = 5): a SHAM and a SCI group killed 24 h after cord transection surgery. The results pointed out genetic damage in blood cells as depicted by the tail moment results. DNA breakage was also detected in liver and kidney cells after SCI. Taken together, our results suggest that SCI induces genomic damage in multiple organs of Wistar rats.
摘要
脊髓损伤（SCI）是一种具有重要功能和心理后果的破坏性疾病。然而，这些改变发生的潜在机制仍不完全清楚。本研究的目的是通过单细胞凝胶（彗星）分析来分析 SCI 急性期多个器官的基因组不稳定性。大鼠随机分为两组（n = 5）：假手术（SHAM）组和脊髓横断手术后 24 小时处死的 SCI 组。结果表明，尾部矩结果显示血细胞存在遗传损伤。SCI 后还检测到肝和肾细胞中的 DNA 断裂。综上所述，我们的结果表明，SCI 诱导 Wistar 大鼠多个器官的基因组损伤。
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