College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.
Neurochem Res. 2012 Jul;37(7):1584-97. doi: 10.1007/s11064-012-0754-9. Epub 2012 Apr 4.
To investigate the protective effects of curcumin against amyloid-β (Aβ)-induced neuronal damage. Primary rat cortical neurons were cultured with different treatments of Aβ and curcumin. Neuronal morphologies, viability and damage were assessed. Neuronal oxidative stress was assessed, including extracellular hydrogen peroxide and intracellular reactive oxygen species. The abilities of curcumin to scavenge free radicals and to inhibit Aβ aggregation and β-sheeted formation are further assessed and discussed. Curcumin preserves cell viability, which is decreased by Aβ. The results of changed morphology, released Lactate dehydrogenases and cell viability assays indicate that curcumin protects Aβ-induced neuronal damage. Curcumin depresses Aβ-induced up-regulation of neuronal oxidative stress. The treatment sequence impacts the protective effect of curcumin on Aβ-induced neuronal damage. Curcumin shows a more protective effect on neuronal oxidative damage when curcumin was added into cultured neurons not later than Aβ, especially prior to Aβ. The abilities of curcumin to scavenge free radicals and to inhibit the formation of β-sheeted aggregation are both beneficial to depress Aβ-induced oxidative damage. Curcumin prevents neurons from Aβ-induced oxidative damage, implying the therapeutic usage for the treatment of Alzheimer's disease patients.
为了研究姜黄素对淀粉样蛋白-β（Aβ）诱导的神经元损伤的保护作用。原代大鼠皮质神经元用不同浓度的 Aβ和姜黄素处理后进行培养。评估神经元形态、活力和损伤情况。评估神经元氧化应激情况，包括细胞外过氧化氢和细胞内活性氧。进一步评估和讨论了姜黄素清除自由基以及抑制 Aβ聚集和β-折叠形成的能力。姜黄素可以保持细胞活力，而 Aβ则会降低细胞活力。形态变化、乳酸脱氢酶释放和细胞活力测定的结果表明，姜黄素可以保护 Aβ诱导的神经元损伤。姜黄素抑制 Aβ诱导的神经元氧化应激上调。处理顺序影响姜黄素对 Aβ诱导的神经元损伤的保护作用。当姜黄素的加入时间不晚于 Aβ，特别是在 Aβ之前，对神经元氧化损伤的保护作用更为明显。姜黄素清除自由基和抑制β-折叠聚集形成的能力都有利于减轻 Aβ诱导的氧化损伤。姜黄素可以预防神经元受到 Aβ诱导的氧化损伤，这暗示了它在治疗阿尔茨海默病患者中的治疗用途。
