Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
J Neurol Sci. 2022 Oct 15;441:120356. doi: 10.1016/j.jns.2022.120356. Epub 2022 Aug 1.
Alzheimer's disease (AD) is a degenerative disorder characterized by the loss of synapses and neurons in the brain, and results in the accumulation of amyloid-based neurotic plaques. Amyloid-β oligomers (AβO) are widely accepted as the main neurotoxin that induces oxidative stress and neuronal loss in AD. In this study, an oxidative stress model of the neuroblastoma SH-SY5Y cell line exposed to AβO was established to simulate an AD cell model. Exposure to AβO significantly reduced the viability of cultured SH-SY5Y cells (p < 0.05) and significantly increased intracellular reactive oxygen species (ROS) (p < 0.01). AβO exposure also induced oxidative stress in SH-SY5Y cells. Furthermore, AβO significantly increased the level of hyperphosphorylation of tau at sites T181 and T205 in SH-SY5Y cells (p < 0.01). Using edaravone, a free radical scavenger with neuroprotective properties, as the control, the possible protective and anti-oxidative effects of curcumin (40 μM) and resveratrol (20 μM) were evaluated. The results suggest that curcumin and resveratrol decreased ROS generation, attenuated oxidative stress, inhibited tau hyperphosphorylation, and protected SH-SY5Y cells from AβO damage. Both curcumin and resveratrol are promising supplements or medicine as therapeutic agents for the treatment of AD.
阿尔茨海默病（AD）是一种退行性疾病，其特征是大脑中突触和神经元的丧失，并导致基于淀粉样蛋白的神经斑块的积累。淀粉样β寡聚物（AβO）被广泛认为是诱导 AD 中氧化应激和神经元丧失的主要神经毒素。在这项研究中，建立了一种暴露于 AβO 的神经母细胞瘤 SH-SY5Y 细胞系的氧化应激模型，以模拟 AD 细胞模型。AβO 暴露显著降低了培养的 SH-SY5Y 细胞的活力（p<0.05），并显著增加了细胞内活性氧（ROS）（p<0.01）。AβO 暴露还诱导了 SH-SY5Y 细胞的氧化应激。此外，AβO 显著增加了 SH-SY5Y 细胞中 tau 丝氨酸 181 和苏氨酸 205 位点的过度磷酸化水平（p<0.01）。使用具有神经保护作用的自由基清除剂依达拉奉作为对照，评估了姜黄素（40 μM）和白藜芦醇（20 μM）的可能保护和抗氧化作用。结果表明，姜黄素和白藜芦醇减少了 ROS 的产生，减轻了氧化应激，抑制了 tau 的过度磷酸化，并保护 SH-SY5Y 细胞免受 AβO 的损伤。姜黄素和白藜芦醇都是治疗 AD 的有前途的补充剂或药物。
