Yamamura Mitsuhiro, Miyamoto Yuji, Mitsuno Masataka, Ohata Toshihiro, Tanaka Hiroe, Kobayashi Yasuhiko, Ryomoto Masaaki, Yoshioka Yoshiteru
Department of Cardiovascular Surgery, Hyogo College of Medicine, Hyogo, Japan.
Int J Angiol. 2007 Spring;16(1):17-9. doi: 10.1055/s-0031-1278238.
The present study evaluated whether the free radical scavenger edaravone (Radicut [Mitsubishi Pharma Co, Japan]) can suppress lower extremity postoperative reperfusion injury by evaluating muscle cell viability with immunohistological stain (cytochrome c oxidase stain).
Eight Lewis male rats (460 g to 510 g) were divided into two groups. In the control group, postoperative reperfusion injury models were created by clamping the bilateral common femoral arteries for 5 h and then releasing. In the other group, 9.0 mg/kg of edaravone was administered before clamping the bilateral common femoral arteries. After 5 h of reperfusion, the bilateral triceps muscles in both groups were stained with cytochrome c oxidase stain (each n=4 × 2). The positive areas of cytochrome c oxidase stain were measured and compared, using computerized densitometry (National Institutes of Health Image program, version 1.61).
In the control group, the lower triceps muscles were not stained with cytochrome c oxidase. In the edaravone group, the lower triceps muscles were strongly stained with cytochrome c oxidase. The positive areas of cytochrome c oxidase stain were significantly greater in the edaravone group (133,000±12,000 μ(2)/mm(2), P<0.01) compared with the control group (8000±1300 μ(2)/mm(2)).
The present study suggests that the preoperative administration of 9.0 mg/kg of edaravone may suppress postoperative reperfusion injury in a rat model.
本研究通过免疫组织化学染色（细胞色素c氧化酶染色）评估肌肉细胞活力，以评价自由基清除剂依达拉奉（Radicut，日本三菱制药公司生产）是否能抑制下肢术后再灌注损伤。
将8只体重460 g至510 g的雄性Lewis大鼠分为两组。对照组通过夹闭双侧股总动脉5小时后再松开，建立术后再灌注损伤模型。另一组在夹闭双侧股总动脉前给予9.0 mg/kg依达拉奉。再灌注5小时后，两组的双侧肱三头肌均用细胞色素c氧化酶染色（每组n = 4×2）。使用计算机密度测定法（美国国立卫生研究院图像程序，版本1.61）测量并比较细胞色素c氧化酶染色的阳性面积。
对照组中，肱三头肌下部未被细胞色素c氧化酶染色。依达拉奉组中，肱三头肌下部被细胞色素c氧化酶强烈染色。与对照组（8000±1300 μ(2)/mm(2)）相比，依达拉奉组细胞色素c氧化酶染色的阳性面积显著更大（133,000±12,000 μ(2)/mm(2)，P<0.01）。
本研究表明，术前给予9.0 mg/kg依达拉奉可能抑制大鼠模型中的术后再灌注损伤。
