Yamamura Mitsuhiro, Miyamoto Yuji, Mitsuno Masataka, Tanaka Hiroe, Ryomoto Masaaki
Department of Cardiovascular Surgery, Hyogo College of Medicine, Hyogo, Japan.
Int J Angiol. 2010 Spring;19(1):e38-40. doi: 10.1055/s-0031-1278366.
Free radicals have been implicated in myonephropathic metabolic syndrome (MNMS), which not only damages muscles but also the kidneys and lungs. It was recently shown that the free radical scavenger edaravone suppressed reperfusion injury in rat extremities. The present study evaluated whether edaravone also protects against MNMS-induced tissue damage in the lungs and kidneys after reperfusion injury of rat extremities.
Ten male Lewis rats (mean [± SD] weight 508±33 g) were divided into two groups. The MNMS models were created by clamping the bilateral common femoral arteries for 5 h, followed by declamping. In another group, 3.0 mg/kg of edaravone was injected into the peritoneal cavity before clamping the bilateral common femoral arteries. Five hours after starting reperfusion, the kidneys and lungs were harvested from each rat for histological study (n=10). Kidney damage was expressed as the number of infiltrating cells in the glomeruli. Lung damage was expressed as the percentage area of the alveolar wall thickness with cellular infiltration, using computerized densitometry.
Kidneys in the edaravone group showed less cellular infiltration than in the control group (62.2±2.4 cells versus 75.8±3.6 cells per glomerulus, respectively; P=0.002). Lungs in the edaravone group also showed a significantly lower percentage of damaged lung tissue area than in the control group (20.5±1.5% versus 63.6±2.8%, respectively; P<0.001).
The results suggest that the free radical scavenger edaravone might protect against kidney and lung damage induced by MNMS after reperfusion injury of rat extremities.
自由基与肌肾病变代谢综合征（MNMS）有关，该综合征不仅会损害肌肉，还会损害肾脏和肺部。最近有研究表明，自由基清除剂依达拉奉可抑制大鼠肢体的再灌注损伤。本研究评估了依达拉奉是否也能保护大鼠肢体再灌注损伤后MNMS诱导的肺和肾组织损伤。
将10只雄性Lewis大鼠（平均体重[±标准差]为508±33 g）分为两组。通过夹闭双侧股总动脉5小时，然后松开夹闭来建立MNMS模型。另一组在夹闭双侧股总动脉前，经腹腔注射3.0 mg/kg依达拉奉。再灌注开始5小时后，从每只大鼠身上采集肾脏和肺组织进行组织学研究（n = 10）。肾损伤以肾小球内浸润细胞数表示。肺损伤以肺泡壁增厚伴细胞浸润的面积百分比表示，采用计算机密度测定法。
依达拉奉组的肾脏细胞浸润少于对照组（分别为每肾小球62.2±2.4个细胞和75.8±3.6个细胞；P = 0.002）。依达拉奉组的肺组织受损面积百分比也显著低于对照组（分别为20.5±1.5%和63.6±2.8%；P < 0.001）。
结果表明，自由基清除剂依达拉奉可能对大鼠肢体再灌注损伤后MNMS诱导的肾和肺损伤具有保护作用。
