秋水仙碱可阻断促胰液素对猪胆管细胞微管小泡和质膜形态的作用，并损害胆小管的HCO3-分泌。

Colchicine blocks the effects of secretin on bile duct cell tubulovesicles and plasma membrane geometry and impairs ductular HCO3- secretion in the pig.

作者信息

Veel T, Buanes T, Grotmol T, Engeland E, Raeder M G

机构信息

Institute for Experimental Medical Research, Ullevaal Hospital, Oslo, Norway.

出版信息

Acta Physiol Scand. 1990 Aug;139(4):603-7. doi: 10.1111/j.1748-1716.1990.tb08965.x.

DOI:10.1111/j.1748-1716.1990.tb08965.x

PMID:2248039

Abstract

Secretin causes the bile duct cells to secrete HCO3-. To examine whether the transformation of duct cell ultrastructure that follows secretin stimulation depends on microtubules and is important for ductular HCO3- secretion, we examined the effect of colchicine on ductular HCO3- secretion and on the morphology of cells lining bile ductules of anaesthetized pigs. Colchicine blocked secretin-dependent cytoplasmic clearance of tubulovesicles and prevented expansion of the basolateral plasma membrane in duct cells and reduced the ductular HCO3- secretory response from 132 +/- 25 mumol min-1 to 97 +/- 14 mumol min-1. In contrast, lumicolchicine did not affect secretin-dependent tubulovesicle clearance or plasma membrane geometry or ductular HCO3- secretion. Accordingly, secretin-dependent cytoplasmic clearance of tubulovesicles in bile duct cells appears to depend on microtubules and to be important for ductular HCO3- secretion.

摘要

促胰液素可使胆管细胞分泌HCO3-。为了研究促胰液素刺激后胆管细胞超微结构的转变是否依赖于微管且对胆管HCO3-分泌至关重要，我们检测了秋水仙碱对麻醉猪胆管HCO3-分泌以及胆管小支管内衬细胞形态的影响。秋水仙碱阻断了促胰液素依赖的微管泡细胞质清除过程，阻止了胆管细胞基底外侧质膜的扩张，并使胆管HCO3-分泌反应从132±25 μmol·min-1降至97±14 μmol·min-1。相比之下，光秋水仙碱并不影响促胰液素依赖的微管泡清除、质膜几何形状或胆管HCO3-分泌。因此，胆管细胞中促胰液素依赖的微管泡细胞质清除似乎依赖于微管，且对胆管HCO3-分泌很重要。