Faculty of Veterinary Medicine, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan.
Res Vet Sci. 2012 Dec;93(3):1512-4. doi: 10.1016/j.rvsc.2012.03.004. Epub 2012 Apr 4.
Newcastle disease (ND) is a highly contagious viral disease and is a continuous threat to the poultry industry worldwide. In the early months of 2011, several devastating ND outbreaks occurred in Jordan affecting broilers, layers and breeders. The fusion gene of the isolated Newcastle disease virus (NDV) was partially amplified by RT-PCR, then directly sequenced. The NDV isolates were found to have the motif112RRQKRF117. This motif and a mean death time (MDT) of 46 h are indicative of the velogenic nature of these NDV isolates. Phylogenetic analysis showed that the new NDV strain belongs to the lineage 5d (Aldous et al., 2003) and is closely related to the Chinese strain SG/Liaoning/2009. NDV outbreaks in 2010 and 2011 have been noted in neighboring countries. Based on the high nucleotide similarity between our isolated NDV isolates and the Chinese NDV strain, the origin of these recent NDV isolates might be from China.
新城疫（ND）是一种高度传染性的病毒性疾病，是全世界家禽业的持续威胁。2011 年初，约旦发生了几起毁灭性的新城疫疫情，影响了肉鸡、蛋鸡和种鸡。通过 RT-PCR 部分扩增了分离的新城疫病毒（NDV）的融合基因，然后直接测序。NDV 分离株具有 motif112RRQKRF117。该motif 和 46 小时的平均死亡时间（MDT）表明这些 NDV 分离株具有强毒力。系统进化分析表明，新的 NDV 株属于 5d 谱系（Aldous 等人，2003），与中国株 SG/Liaoning/2009 密切相关。2010 年和 2011 年，邻国也出现了 NDV 疫情。基于我们分离的 NDV 分离株与中国 NDV 株之间的高核苷酸相似性，这些最近的 NDV 分离株的起源可能来自中国。
