Department of Microbiology, Mount Sinai School of Medicine, New York, New York, USA.
J Virol. 2012 Oct;86(19):10852-6. doi: 10.1128/JVI.01298-12. Epub 2012 Jul 11.
Paramyxoviruses produce pleiomorphic particles containing variable amounts of genetic material that correlate with virion diameter by electron microscopy. However, the infectious nature of these particles is unknown, and functional genomes are indistinguishable from defective RNA. A quantitative approach to paramyxovirus packaging revealed a majority of infectious Newcastle disease viruses contain one functional genome. Virions encapsidating two or three genomes (approximately 25% of total) were also observed by utilizing three different recombinant viruses expressing unique fluorescent reporters.
副粘病毒产生形态多样的粒子，其中包含数量可变的遗传物质，这些物质通过电子显微镜与病毒直径相关。然而，这些粒子的感染性尚不清楚，并且功能基因组与缺陷 RNA 无法区分。一种定量研究副粘病毒包装的方法表明，大多数具有感染性的新城疫病毒仅含有一个功能基因组。通过利用三种表达独特荧光报告基因的重组病毒，也观察到了包装两个或三个基因组（约占总数的 25%）的病毒粒子。
