1-(4-甲基苄基)-2-对甲苯-1H-苯并[d]咪唑与牛血清白蛋白的结合相互作用。
Binding interaction of 1-(4-methybenzyl)-2-p-tolyl-1H-benzo[d]imidazole with bovine serum albumin.
机构信息
Department of Chemistry, Annamalai University, Annamalainagar, Tamilnadu 608002, India.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2012 Jul;93:180-4. doi: 10.1016/j.saa.2012.03.018. Epub 2012 Mar 15.
A promising benzimidazole derivative 1-(4-methybenzyl)-2-p-tolyl-1H-benzo[d]imidazole (MBTBI) has been synthesized and characterized by single crystal XRD, NMR, mass and IR spectral techniques. The mutual interaction of this benzimidazole derivative (MBTBI) with bovine serum albumin (BSA) was investigated using solution spectral studies. The fluorescence quenching mechanism of BSA by MBTBI was analyzed and the binding constant has been calculated. The binding distance between these two was obtained based on the theory of Forester's non-radiation energy transfer (FRET). The effect of some common ions on the binding constant was also examined.
一种有前途的苯并咪唑衍生物 1-(4-甲基苄基)-2-对甲苯-1H-苯并[d]咪唑（MBTBI）已通过单晶 X 射线衍射、NMR、质谱和红外光谱技术进行了合成和表征。使用溶液光谱研究研究了该苯并咪唑衍生物（MBTBI）与牛血清白蛋白（BSA）的相互作用。分析了 MBTBI 使 BSA 荧光猝灭的机制，并计算了结合常数。基于 Forester 的非辐射能量转移（FRET）理论，获得了这两种物质之间的结合距离。还检查了一些常见离子对结合常数的影响。
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