Cui Gaofeng, Botuyan Maria Victoria, Mer Georges
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA.
Biomol NMR Assign. 2013 Apr;7(1):79-83. doi: 10.1007/s12104-012-9382-1. Epub 2012 Apr 6.
The LOTUS or OST-HTH domain is a recently discovered motif of about 80 amino acids and is found in several germline-specific proteins including the Tudor domain-containing proteins TDRD5 and TDRD7, which are important for germ cell development. The LOTUS domain is an RNA binding domain but its exact function is unknown. Here, we report the (1)H, (13)C and (15)N resonance assignments for the three LOTUS domains present in mouse TDRD7. These assignments will allow three-dimensional structure determination of the LOTUS domains and mapping of their interaction with RNA, steps toward deciphering the function of TDRD7.
LOTUS或OST-HTH结构域是最近发现的一种约含80个氨基酸的基序，存在于几种生殖系特异性蛋白中，包括含Tudor结构域的蛋白TDRD5和TDRD7，它们对生殖细胞发育很重要。LOTUS结构域是一个RNA结合结构域，但其确切功能尚不清楚。在此，我们报道了小鼠TDRD7中存在的三个LOTUS结构域的（1）H、（13）C和（15）N共振归属。这些归属将有助于确定LOTUS结构域的三维结构及其与RNA相互作用的图谱，这是解读TDRD7功能的重要步骤。
