结直肠癌患者正常组织与肿瘤组织中MGMT基因表达的评估。
Evaluation of the expression of the MGMT gene in normal and neoplastic tissue of patients with colorectal cancer.
作者信息
Cordeiro Adriana Teixeira, Silva Camila Morais Gonçalves da, Bartchewsky Júnior Waldemar, Ribeiro Marcelo Lima, Martinez Carlos Augusto Real
机构信息
Universidade São Francisco, Bragança Paulista, SP, Brazil.
出版信息
Rev Col Bras Cir. 2012;39(1):48-53.
OBJECTIVE
To evaluate the expression of tissue repair gene MGMT by comparing normal and neoplastic colonic mucosa in patients with colorectal cancer (CRC).
METHODS
We studied 44 patients with colorectal cancer confirmed by histopathology. We excluded patients suspected of belonging to families with hereditary colorectal cancer (HNPCC and FAP) and patients with cancer of the lower or medium rectum treated with neoadjuvant chemoradiotherapy. The MGMT gene expression was assessed by the technique of polymerase chain reaction in real time (RT-PCR). The comparison of results for MGMT gene expression between normal and neoplastic tissues was made by paired Student's t test, adopting a significance level of 5% (p <0.05).
RESULTS
Tissue expression of the MGMT gene in all patients was lower in tumor tissue when compared to normal tissue (p = 0.002).
CONCLUSION
The repair gene MGMT is less expressed in tumor tissue compared to normal tissues in patients with sporadic CRC.
目的
通过比较结直肠癌（CRC）患者的正常结肠黏膜和肿瘤性结肠黏膜，评估组织修复基因O6-甲基鸟嘌呤-DNA甲基转移酶（MGMT）的表达情况。
方法
我们研究了44例经组织病理学确诊的结直肠癌患者。我们排除了疑似遗传性结直肠癌家族（遗传性非息肉病性结直肠癌和家族性腺瘤性息肉病）的患者以及接受新辅助放化疗的中低位直肠癌患者。采用实时聚合酶链反应（RT-PCR）技术评估MGMT基因表达。通过配对t检验比较正常组织和肿瘤组织中MGMT基因表达的结果，显著性水平为5%（p<0.05）。
结果
与正常组织相比，所有患者肿瘤组织中MGMT基因的组织表达均较低（p=0.002）。
结论
在散发性结直肠癌患者中，与正常组织相比，修复基因MGMT在肿瘤组织中的表达较低。
Zotero 插件