Ren Li-Fen, Ma Yue-Yun, Yue Qiao-Hong, Su Ming-Quan, Hao Xiao-Ke
Center for Clinical Laboratory Medicine of People's Liberation Army, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Apr;28(4):347-9.
To investigate the effect of TFDP3 on prostate cancer cell line LNCaP by transgenic method, and to explore the effect of TFDP3 on regulating the autophay and apoptosis by co-regulation with E2F1.
LNCaP cells were transfected with pcDNA3.1-TFDP3, pCMV-E2F1-HA or pcDNA3.1 empty vector.The expression of TFDP3, E2F1 and LC3B were detected by real-time PCR after transfection for 24 h. Western blotting was used to monitor the changes in autophagy-associated protein LC3B, Apoptosis of transfected cells were analyzed by flow cytometry.
The results showed that activation of TFDP3 upregulates the expression of autophagy genes-microtubule-associated protein-1 light chain-3B (LC3B), and E2F1 antagonizes TFDP3-induced autophagy, and TFDP3 can inhibit E2F1-induced apoptosis.
TFDP3 upregulates the expression of autophagy gene LC3B and inhibits E2F1-induced apoptosis, and may play an important role in prostate cancer.
通过转基因方法研究TFDP3对前列腺癌细胞系LNCaP的影响，并探讨TFDP3与E2F1共同调节对自噬和凋亡的影响。
用pcDNA3.1-TFDP3、pCMV-E2F1-HA或pcDNA3.1空载体转染LNCaP细胞。转染24小时后，通过实时PCR检测TFDP3、E2F1和LC3B的表达。采用蛋白质免疫印迹法监测自噬相关蛋白LC3B的变化，通过流式细胞术分析转染细胞的凋亡情况。
结果显示，TFDP3的激活上调了自噬基因微管相关蛋白1轻链3B（LC3B）的表达，E2F1拮抗TFDP3诱导的自噬，且TFDP3可抑制E2F1诱导的凋亡。
TFDP3上调自噬基因LC3B的表达并抑制E2F1诱导的凋亡，可能在前列腺癌中发挥重要作用。
