[TFDP3对LNCaP细胞自噬和凋亡的调控作用]
[Effects of TFDP3 on regulating the autophagy and apoptosis of LNCaP cells].
作者信息
Ren Li-Fen, Ma Yue-Yun, Yue Qiao-Hong, Su Ming-Quan, Hao Xiao-Ke
机构信息
Center for Clinical Laboratory Medicine of People's Liberation Army, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Apr;28(4):347-9.
AIM
To investigate the effect of TFDP3 on prostate cancer cell line LNCaP by transgenic method, and to explore the effect of TFDP3 on regulating the autophay and apoptosis by co-regulation with E2F1.
METHODS
LNCaP cells were transfected with pcDNA3.1-TFDP3, pCMV-E2F1-HA or pcDNA3.1 empty vector.The expression of TFDP3, E2F1 and LC3B were detected by real-time PCR after transfection for 24 h. Western blotting was used to monitor the changes in autophagy-associated protein LC3B, Apoptosis of transfected cells were analyzed by flow cytometry.
RESULTS
The results showed that activation of TFDP3 upregulates the expression of autophagy genes-microtubule-associated protein-1 light chain-3B (LC3B), and E2F1 antagonizes TFDP3-induced autophagy, and TFDP3 can inhibit E2F1-induced apoptosis.
CONCLUSION
TFDP3 upregulates the expression of autophagy gene LC3B and inhibits E2F1-induced apoptosis, and may play an important role in prostate cancer.
目的
通过转基因方法研究TFDP3对前列腺癌细胞系LNCaP的影响，并探讨TFDP3与E2F1共同调节对自噬和凋亡的影响。
方法
用pcDNA3.1-TFDP3、pCMV-E2F1-HA或pcDNA3.1空载体转染LNCaP细胞。转染24小时后，通过实时PCR检测TFDP3、E2F1和LC3B的表达。采用蛋白质免疫印迹法监测自噬相关蛋白LC3B的变化，通过流式细胞术分析转染细胞的凋亡情况。
结果
结果显示，TFDP3的激活上调了自噬基因微管相关蛋白1轻链3B（LC3B）的表达，E2F1拮抗TFDP3诱导的自噬，且TFDP3可抑制E2F1诱导的凋亡。
结论
TFDP3上调自噬基因LC3B的表达并抑制E2F1诱导的凋亡，可能在前列腺癌中发挥重要作用。
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