Research Group for Preventive Technology in Swine and Ruminant Disease, Khon Kaen University, Thailand.
Anim Reprod Sci. 2012 Apr;131(3-4):194-8. doi: 10.1016/j.anireprosci.2012.03.003. Epub 2012 Mar 11.
Levels of the cytokines transforming growth factor (TGF)-β1, interleukin (IL)-10 and IL-6 in the boar seminal plasma (SP) as well as TGF-β1 level in different fractions of ejaculate were studied. These cytokines was chosen because of their expected effect on tissue immune response, i.e. suppressive (TGF-β1 and IL-10) and pro-inflammatory (IL-6). Three whole ejaculates from five boars A-E, (n=15) were sampled weekly to evaluate the levels of seminal plasma TGF-β1, IL-10 and IL-6 as well as their fluctuations over time. The effect of different storage temperatures, -20°C or -80°C, on the level of seminal plasma TGF β1 was also tested (three boars, two fractions in one ejaculate). In addition, in 4 different fractions of ejaculates: the pre-sperm-rich (Pre-SRF), first 10 ml of sperm-rich (10SRF), the rest of the sperm-rich fraction (Rest-SRF) and the rest of the ejaculate (RE) fraction, were collected from three boars (A-C) on four different occasions for TGF-β1 evaluation. In the whole ejaculates (n=15), a wide range in the concentration of the cytokines TGF-β1 (20.4 - 766.5 pg/mL) and IL-10, (73.7 - 837.3 pg/mL), was found. For IL-6, the concentration was low (range 11.5 - 30.9 pg/ml) and only detected in four out of 15 collections (from two boars). The mean levels of TGF-β1 and IL-10 between individual boars varied but were not statistical different. The level of TGF-β1 in Pre-SRF, Rest-SRF and RE fractions was significantly lower in boar A than the other boars. A significantly higher concentration of TGF-β1 was found in the 10SRF than in the other fractions. Different storage temperatures (-20°C or -80°C) did not affect the seminal plasma TGF-β1 level after one year of storage. To conclude: Boar seminal plasma contained TGF- β1 and IL-10 but with high individual variation. IL-6 was low or undetectable. The TGF- β1 level was highest in the first 10 mL of the sperm-rich fraction of the ejaculate. Further studies are needed on the role of different levels of cytokine in boar semen on porcine female reproductive tissue, especially for TGF- β1.
本研究旨在探讨公猪精液中细胞因子转化生长因子 (TGF)-β1、白细胞介素 (IL)-10 和 IL-6 的水平，以及不同精液成分中 TGF-β1 的水平。选择这些细胞因子是因为它们预计会对公猪组织免疫反应产生影响，即抑制性 (TGF-β1 和 IL-10) 和促炎性 (IL-6)。从 5 头公猪 A-E 中每周采集 3 次整个精液样本，以评估精液中 TGF-β1、IL-10 和 IL-6 的水平及其随时间的波动。还测试了不同储存温度（-20°C 或-80°C）对精液 TGF-β1 水平的影响（3 头公猪，每个精液样本的 2 个部分）。此外，从 3 头公猪（A-C）的 4 个不同精液部分（富含前精子的部分[Pre-SRF]、前 10ml 富含精子的部分[10SRF]、其余富含精子的部分[Rest-SRF]和其余精液部分[RE]）收集了 TGF-β1 进行评估。在整个精液样本（n=15）中，TGF-β1（20.4-766.5 pg/mL）和 IL-10（73.7-837.3 pg/mL）的浓度范围很广。IL-6 的浓度较低（范围为 11.5-30.9 pg/ml），仅在 15 次采集中的 4 次中检测到（来自 2 头公猪）。个体公猪之间 TGF-β1 和 IL-10 的平均水平存在差异，但无统计学差异。富含前精子的部分、其余富含精子的部分和其余精液部分中 TGF-β1 的水平在公猪 A 中明显低于其他公猪。10SRF 中的 TGF-β1 浓度明显高于其他部分。储存一年后，不同的储存温度（-20°C 或-80°C）对精液 TGF-β1 水平没有影响。综上所述：公猪精液中含有 TGF-β1 和 IL-10，但个体差异较大。IL-6 含量较低或无法检测到。TGF-β1 水平在精液中富含精子的前 10ml 部分最高。需要进一步研究不同水平的细胞因子在公猪精液对猪雌性生殖组织的作用，特别是对 TGF-β1 的作用。
