Barranco Isabel, Rubér Marie, Perez-Patiño Cristina, Atikuzzaman Mohammad, Martinez Emilio A, Roca Jordi, Rodriguez-Martinez Heriberto
Department of Medicine & Animal Surgery, University of Murcia, Murcia, Spain.
Department of Clinical & Experimental Medicine, University of Linköping, Linköping, Sweden.
Am J Reprod Immunol. 2015 Dec;74(6):523-32. doi: 10.1111/aji.12432. Epub 2015 Sep 28.
The boar, as human, sequentially ejaculates sperm-rich and sperm-poor fractions. Seminal plasma (SP) spermadhesins (PSP-I/PSP-II) induce a primary endometrial inflammatory response in female sows, similar to that elicited by semen deposition in other species, including human. However, the SP is also known to mitigate such response, making it transient to allow for embryo entry to a cleansed endometrium. Although cytokine involvement has been claimed, the exploration of cytokines in different SP fractions is scarce. This study determines Th1, Th2, Th17 and Th3 cytokine profiles in specific ejaculate SP fractions from boars of proven fertility.
SP samples from the sperm-rich fraction (SRF) and the sperm-poor post-SRF fraction (post-SRF) of manually collected ejaculates from eight boars (four ejaculates per boar) were analysed by commercial multiplex bead assay kits (Milliplex MAP, Millipore, USA) for interferon-γ, interferon gamma-induced protein 10, macrophage-derived chemokine, growth-regulated oncogene, granulocyte-macrophage colony-stimulating factor, monocyte chemo-attractant protein-1, interleukins (IL)-6, IL-8, IL-10, IL-15, IL-17 and transforming growth factor (TGF)-β1-β3.
Cytokine concentrations differed between the ejaculate fractions among boars, being highest in the post-SRF.
Boar SP is rich in Th1, Th2, Th17 and Th3 cytokines, with lowest concentrations in the sperm-peak-containing fraction, indicating its main immune influence might reside in the larger, protein-rich sperm-poor post-SRF.
公猪与人类一样，射精过程会依次排出富含精子和精子较少的部分。精浆（SP）中的精子黏附素（PSP-I/PSP-II）会在母猪子宫内膜引发原发性炎症反应，这与包括人类在内的其他物种精液沉积所引发的反应类似。然而，已知精浆也能减轻这种反应，使其具有短暂性，以便胚胎能够进入清洁的子宫内膜。尽管有人认为细胞因子参与其中，但对不同精浆部分中细胞因子的研究却很少。本研究确定了来自具有生育能力的公猪特定射精精浆部分中的Th1、Th2、Th17和Th3细胞因子谱。
使用商业多重微珠检测试剂盒（Milliplex MAP，美国密理博公司），对8头公猪（每头公猪采集4次射精样本）手动采集的射精样本中富含精子部分（SRF）和SRF之后精子较少部分（post-SRF）的精浆样本进行分析，检测干扰素-γ、干扰素γ诱导蛋白10、巨噬细胞衍生趋化因子、生长调节致癌基因、粒细胞-巨噬细胞集落刺激因子、单核细胞趋化蛋白-1、白细胞介素（IL）-6、IL-8、IL-10、IL-15、IL-17以及转化生长因子（TGF）-β1-β3。
不同公猪射精部分之间的细胞因子浓度存在差异，post-SRF部分中的细胞因子浓度最高。
公猪精浆富含Th1、Th2、Th17和Th3细胞因子，在含有精子峰值的部分中浓度最低，这表明其主要免疫影响可能存在于体积较大、富含蛋白质的post-SRF部分。
