子宫内膜异位症患者基质细胞中缺氧诱导因子-1α对雌激素受体表达的影响。
Estrogen receptor expression affected by hypoxia inducible factor-1α in stromal cells from patients with endometriosis.
机构信息
Department of Obstetrics and Gynecology, College of Medicine and Hospital, National Cheng Kung University, Tainan, Taiwan.
出版信息
Taiwan J Obstet Gynecol. 2012 Mar;51(1):50-4. doi: 10.1016/j.tjog.2012.01.010.
OBJECTIVE
Endometriosis is an estrogen-dependent disease. The aim of this study was to evaluate the different expression of estrogen receptors (ER) and its relation to hypoxia inducible factor-1α (HIF-1α) in stromal cells from women with endometriosis.
MATERIALS AND METHODS
Paired eutopic endometrial and ectopic endometriotic stromal cells were isolated from women with endometriosis while they underwent laparoscopy. The expression of ERα and ERβ was measured by reverse transcription-polymerase chain reaction and Western blot. Regulation of ER expression was evaluated by HIF-1α knockdown via short interference RNA.
RESULTS
The expression of ERβ was significantly increased in ectopic stromal cells. Treatment of endometrial stromal cells with hypoxia induced ERβ expression. Knockdown of HIF-1α abolished hypoxia-induced ERβ expression and increased ERα expression.
CONCLUSION
The expression of ERβ is regulated by hypoxia. Results of this study will provide important information in the involvement of hypoxia factors in mediating estrogen action via different ER expression in endometriosis.
目的
子宫内膜异位症是一种雌激素依赖性疾病。本研究旨在评估子宫内膜异位症患者间质细胞中雌激素受体（ER）的不同表达及其与缺氧诱导因子-1α（HIF-1α）的关系。
材料和方法
在腹腔镜检查期间，从子宫内膜异位症患者中分离出配对的在位子宫内膜和异位子宫内膜间质细胞。通过逆转录聚合酶链反应和 Western blot 检测 ERα和 ERβ的表达。通过短干扰 RNA 敲低 HIF-1α 评估 ER 表达的调节。
结果
ERβ在异位间质细胞中的表达显著增加。缺氧处理子宫内膜间质细胞诱导 ERβ表达。HIF-1α 的敲低消除了缺氧诱导的 ERβ表达并增加了 ERα 的表达。
结论
ERβ的表达受缺氧调节。本研究的结果将为缺氧因子通过不同 ER 表达在子宫内膜异位症中介导雌激素作用提供重要信息。
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