Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, Jilin University, Changchun 130012, China.
J Hazard Mater. 2012 May 30;217-218:156-63. doi: 10.1016/j.jhazmat.2012.03.008. Epub 2012 Mar 10.
Blood is a waste product of the slaughter industry, while its main component hemoglobin (Hb) is a pseudo-peroxidase which is able to oxidize polycyclic aromatic hydrocarbons (PAHs) in the presence of H(2)O(2). In order to use Hb for wastewater treatment, we encapsulated it in silica-based matrix by modified "fish-in-net" approach. The as-synthesized catalysts were characterized by SEM, TEM, BET and solid-state UV-vis spectroscopy. It was found that Hb was partially homogeneously dispersed in microspheres and showed more stable peroxidase-like activity than free Hb. Moreover, it had substantially increased storage stability as well as pH stability. It was used as biocatalyst to remove aniline in aqueous solution and gave a reduction of 65% aniline removal, while 76% in the presence of additive PEG. No significant activity loss was observed after ten runs. These experimental results suggest that the resultant product was a promising biocatalyst for aromatic wastewater treatment.
血液是屠宰业的废物产物，而其主要成分血红蛋白（Hb）是一种拟过氧化物酶，能够在 H(2)O(2)存在下氧化多环芳烃（PAHs）。为了将 Hb 用于废水处理，我们通过改良的“网中捕鱼”方法将其封装在基于硅的基质中。通过 SEM、TEM、BET 和固态 UV-vis 光谱对合成的催化剂进行了表征。结果发现，Hb 部分均匀地分散在微球中，表现出比游离 Hb 更稳定的过氧化物酶样活性。此外，它的储存稳定性和 pH 稳定性都有了显著提高。它被用作生物催化剂来去除水溶液中的苯胺，在添加添加剂 PEG 的情况下，去除率达到了 65%。经过十次运行后，没有观察到明显的活性损失。这些实验结果表明，所得产物是一种有前途的用于处理芳香族废水的生物催化剂。
