Dong Mengxing, Wu Zhuofu, Lu Ming, Wang Zhi, Li Zhengqiang
Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, China.
Int J Mol Sci. 2012;13(9):11443-11454. doi: 10.3390/ijms130911443. Epub 2012 Sep 12.
Aminopropyl-functionalized SBA-15 mesoporous silica was used as a support to adsorb myoglobin. Then, in order to avoid the leakage of adsorbed myoglobin, lysozyme was covalently tethered to the internal and external surface of the mesoporous silica with glutaraldehyde as the coupling agent. The property of amino-functionalized mesoporous silica was characterized by N(2) adsorption-desorption and thermogravimetric (TG) analysis. The feature of the silica-based matrix before and after myoglobin adsorption was identified by fourier transform infrared (FTIR) and UV/VIS measurement. With o-dianisidine and H(2)O(2) as the substrate, the peroxidase activity of adsorbed myoglobin was determined. With Micrococus lysodeilicus as the substrate, the antibacterial activity of covalently tethered lysozyme was measured. Results demonstrated that the final product not only presented peroxidase activity of the myoglobin but yielded antibacterial activity of the lysozyme.
氨基丙基功能化的SBA-15介孔二氧化硅用作吸附肌红蛋白的载体。然后，为避免吸附的肌红蛋白泄漏，以戊二醛作为偶联剂，将溶菌酶共价连接到介孔二氧化硅的内表面和外表面。通过N₂吸附-脱附和热重（TG）分析对氨基功能化介孔二氧化硅的性质进行了表征。通过傅里叶变换红外（FTIR）和紫外/可见光谱测量确定了吸附肌红蛋白前后硅基基质的特征。以邻联茴香胺和H₂O₂为底物，测定了吸附的肌红蛋白的过氧化物酶活性。以溶壁微球菌为底物，测定了共价连接的溶菌酶的抗菌活性。结果表明，最终产物不仅呈现出肌红蛋白的过氧化物酶活性，还产生了溶菌酶的抗菌活性。
