State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, High-tech District, Dalian 116024, China.
Talanta. 2012 May 15;93:55-61. doi: 10.1016/j.talanta.2012.01.024. Epub 2012 Feb 2.
A new fluorescent and colorimetric probe RCu1 with ultra-sensitivity (detection limit is 4.7 nM (3σ)) was developed. It is based on a rhodamine B derivative modified with a functionalized 8-hydroxyquinoline group as a copper-binding site which greatly increases the affinity for Cu(2+). Cu(2+) acts not only as a selective recognizing guest but also a hydrolytic promoter. RCu1 showed single-selectively sensing Cu(2+) over other cations and much larger fluorescence enhancement (as high as over 1000-fold) than those based on Cu(2+)-complexation. The detection mechanism was proved by TOF-MS, FT-IR, (1)H NMR and Gaussian calculations. Based on the outstanding recognition ability of RCu1 toward Cu(2+), an analytical procedure was developed for Cu(2+)-determination in natural water samples and soil sample.
一种新的超灵敏荧光比色探针 RCu1 被开发出来。它基于一个芴酮衍生物，用功能化的 8-羟基喹啉基团修饰作为铜结合位点，大大增加了对 Cu(2+)的亲和力。Cu(2+)不仅作为选择性识别客体，而且作为水解促进剂。RCu1 对 Cu(2+)表现出单一选择性识别，其荧光增强（高达 1000 倍以上）比基于 Cu(2+)-络合的要强得多。通过飞行时间质谱（TOF-MS）、傅里叶变换红外光谱（FT-IR）、（1）H NMR 和高斯计算证明了检测机制。基于 RCu1 对 Cu(2+)的出色识别能力，开发了一种用于测定天然水样和土壤样品中 Cu(2+)的分析方法。
