Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, Shaanxi, 710127, People's Republic of China.
J Fluoresc. 2019 Mar;29(2):425-434. doi: 10.1007/s10895-019-02351-x. Epub 2019 Feb 6.
A new "turn-on" fluorescent probe Py based on rhodamine and piperonaldehyde was designed and synthesized for detecting Fe in cells. The free probe Py was non-fluorescent. While only upon addition of Fe, the significant increase of the fluorescence and color were observed which could be visible directly by "naked-eye". The probe Py shows high selectivity and sensitivity for Fe over other common metal ions in EtOH-HO (3/2, v/v) mixed solution. The association constant and the detection limit were calculated to be 4.81 × 10 M and 1.18 × 10 mol/L respectively. The introduction of piperonaldehyde unit could increase probe rigidity which could enhance its optical properties. Meanwhile, the binding mode between Py and Fe was found to be a 1:1 complex formation. The density functional theory (DFT) calculations were performed which would further confirm the recognition mechanism between probe Py and Fe. In addition, the probe has been proved to be reversible for detecting Fe. Moreover, the probe Py was used to detect Fe in cells successfully.
一种新的基于罗丹明和香豆素醛的“开”式荧光探针 Py 被设计并合成用于检测细胞中的 Fe。游离探针 Py 没有荧光。只有当加入 Fe 时，才会观察到荧光和颜色的显著增加，这可以通过“肉眼”直接观察到。探针 Py 在 EtOH-HO（3/2，v/v）混合溶液中对 Fe 具有高选择性和灵敏度，而对其他常见金属离子则没有。计算得到的结合常数和检测限分别为 4.81×10^4 M 和 1.18×10^-6 mol/L。香豆素醛单元的引入可以增加探针的刚性，从而增强其光学性质。同时，发现 Py 与 Fe 之间的结合模式为 1:1 络合形成。进行了密度泛函理论（DFT）计算，进一步证实了探针 Py 与 Fe 之间的识别机制。此外，该探针已被证明可用于可逆检测 Fe。此外，探针 Py 已成功用于细胞中 Fe 的检测。
