A.E. Arbuzov Institute of Organic and Physical Chemistry of KSC of RAS, Arbuzov Street, 8, Kazan, Russia.
Talanta. 2012 May 15;93:233-8. doi: 10.1016/j.talanta.2012.02.023. Epub 2012 Feb 17.
The work represents colloids of silica nanoparticles displaying fluorescent response on biorelevant compounds exemplified by phosphacoumarins and phospholipids. The luminescent properties of the colloids arise from Tb(III) complexes doped into silica nanoparticles (SNs). The noncovalent decoration of SNs by dicationic surfactant with further interfacial binding of dye anions enables to develop colloids programmed to display a substrate induced fluorescent response. The latter results from the quenching of Tb(III) centered luminescence by dye anions through dynamic mechanism and subsequent displacement of quenching anions by the non-quenching substrates from the interface of SNs. Both negative charge and hydrophobicity of substrates are the key factors affecting the selectivity of the substrate induced fluorescent response. The peculiar effects of zwitter-ionic and anionic phospholipids on the fluorescent response have been revealed. The applicability of the fluorescent procedure in the sensing of impurities in commercial phosphatidylcholine is also introduced.
该工作展示了在生物相关化合物（如磷酰香豆素和磷脂）存在下显示荧光响应的二氧化硅纳米粒子胶体。胶体的发光性质源于掺杂在二氧化硅纳米粒子（SNs）中的铽（III）配合物。带有进一步界面结合染料阴离子的二价阳离子表面活性剂对 SNs 的非共价修饰，使得开发出的胶体可以显示出由底物诱导的荧光响应。这种荧光响应源于通过动态机制由染料阴离子猝灭铽（III）中心的发光，随后非猝灭底物从 SNs 的界面取代猝灭阴离子。底物的负电荷和疏水性均是影响底物诱导荧光响应选择性的关键因素。还揭示了两性离子和阴离子磷脂对荧光响应的特殊影响。该荧光程序在检测商业磷脂酰胆碱中的杂质方面的适用性也被介绍。
