Epigenomics Project, Institute of Systems and Synthetic Biology, CNRS, University of Evry, France.
FEBS Lett. 2012 Jul 16;586(15):2043-8. doi: 10.1016/j.febslet.2012.03.051. Epub 2012 Mar 31.
Recently the mismatch between our newly acquired capacity to synthetize DNA at genome scale, and our low capacity to design ab initio a functional genome has become conspicuous. This essay gathers a variety of constraints that globally shape natural genomes, with a focus on eubacteria. These constraints originate from chromosome replication (leading/lagging strand asymmetry; gene dosage gradient from origin to terminus; collisions with the transcription complexes), from biased codon usage, from noise control in gene expression, and from genome layout for co-functional genes. On the basis of this analysis, lessons are drawn for full genome design.
最近，我们在基因组规模上合成 DNA 的新能力与从头设计功能基因组的低能力之间的不匹配变得很明显。本文收集了各种全局塑造自然基因组的限制因素，重点是真细菌。这些限制因素源于染色体复制（前导/滞后链不对称；从起点到终点的基因剂量梯度；与转录复合物的碰撞）、偏倚密码子使用、基因表达中的噪声控制以及共功能基因的基因组布局。在此基础上，为全基因组设计汲取了教训。
