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实验室条件下受氨和微囊藻毒素共同作用下沉水植物苦草幼苗的时间依赖性氧化应激响应。

Time-dependent oxidative stress responses of submerged macrophyte Vallisneria natans seedlings exposed to ammonia in combination with microcystin under laboratory conditions.

机构信息

Jiangsu Province Key Laboratory for Biodiversity and Biotechnology, School of Biological Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing, 210046, China.

出版信息

Bull Environ Contam Toxicol. 2012 Jul;89(1):67-72. doi: 10.1007/s00128-012-0633-8. Epub 2012 Apr 8.

DOI:10.1007/s00128-012-0633-8
PMID:22484329
Abstract

We studied the antioxidant responses of macrophyte Vallisnerria natans seedlings to combined ammonia (0, 0.21, and 0.85 mg L(-1)) and microcystin-LR (MC-LR) (0, 10, and 50 μg L(-1)) for 7 days. Results showed that superoxide dismutase, catalase (CAT), peroxidase, and glutathione were significantly induced by the mixtures of ammonia and MC-LR, and there were significant interactions between ammonia and MC-LR. Specially, CAT increased about fivefold at ammonia 0.85 mg L(-1) and MC-LR 50 μg L(-1) on day 3. Malondialdehyde fluctuated with both ammonia and MC-LR, and significant interactions were detected between the two stressors. Changes in all the measured variables were time-dependent.

摘要

我们研究了氨(0、0.21 和 0.85mg/L)和微囊藻毒素-LR(MC-LR)(0、10 和 50μg/L)组合对沉水植物苦草幼苗抗氧化响应的影响,为期 7 天。结果表明,超氧化物歧化酶、过氧化氢酶(CAT)、过氧化物酶和谷胱甘肽均受氨和 MC-LR 的混合物诱导,氨和 MC-LR 之间存在显著的相互作用。特别是,CAT 在第 3 天氨浓度为 0.85mg/L 和 MC-LR 浓度为 50μg/L 时增加了约五倍。丙二醛随氨和 MC-LR 而波动,两种胁迫因子之间存在显著的相互作用。所有测量变量的变化均与时间有关。

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