Hernández-Benítez Reyna, Ramos-Mandujano Gerardo, Pasantes-Morales Herminia
División de Neurociencias, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, Mexico.
Stem Cell Res. 2012 Jul;9(1):24-34. doi: 10.1016/j.scr.2012.02.004. Epub 2012 Mar 7.
This study reports an effect of taurine (1-10 mM) increasing markedly (120%) the number of neural precursor cells (NPCs) from adult mouse subventricular zone, cultured as neurospheres. This effect is one of the highest reported for adult neural precursor cells. Taurine-containing cultures showed 73-120% more cells than controls, after 24 and 96 h in culture, respectively. Taurine effect is due to enhanced proliferation as assessed by BrdU incorporation assays. In taurine cultures BrdU incorporation was markedly higher than controls from 1.5 to 48 h, with the maximal difference found at 1.5 h. This effect of taurine reproduced at every passage with the same window time. Taurine effects are not mimicked by glycine, alanine or GABA. Clonal efficiency values of 3.6% for taurine cultures and 1.3% for control cultures suggest a taurine influence on both, progenitor and stem cells. Upon differentiation, the proportion of neurons in control and taurine cultures was 3.1% (±0.5) and 10.2% (±0.8), respectively. These results are relevant for taurine implication in brain development as well as in adult neurogenesis. Possible mechanisms underlying taurine effects on cell proliferation are discussed.
本研究报告了牛磺酸（1-10 mM）对成年小鼠脑室下区神经前体细胞（NPCs）数量的显著影响，这些细胞培养为神经球时数量增加了120%。这一效应是已报道的对成年神经前体细胞影响最大的之一。含牛磺酸的培养物在培养24小时和96小时后，分别比对照组多73%-120%的细胞。通过BrdU掺入试验评估，牛磺酸的作用是由于增殖增强。在牛磺酸培养物中，从1.5小时到48小时，BrdU掺入量明显高于对照组，在1.5小时时差异最大。牛磺酸的这种作用在每次传代时都在相同的时间窗口重现。甘氨酸、丙氨酸或GABA不能模拟牛磺酸的作用。牛磺酸培养物的克隆效率值为3.6%，对照培养物为1.3%，这表明牛磺酸对祖细胞和干细胞都有影响。在分化时，对照培养物和牛磺酸培养物中神经元的比例分别为3.1%（±0.5）和10.2%（±0.8）。这些结果与牛磺酸在脑发育以及成体神经发生中的作用有关。文中还讨论了牛磺酸对细胞增殖影响的潜在机制。
