Weigel Detlef, Glazebrook Jane
CSH Protoc. 2006 Dec 1;2006(7):pdb.prot4666. doi: 10.1101/pdb.prot4666.
INTRODUCTIONThis protocol describes a method for transforming Agrobacterium with plasmid DNA using a freeze-thaw technique. Although the transformation efficiency for Agrobacterium is lower than that for Escherichia coli, it is possible to obtain adequate numbers of transformants with this technique.
引言
本方案描述了一种使用冻融技术用质粒DNA转化农杆菌的方法。虽然农杆菌的转化效率低于大肠杆菌，但用该技术有可能获得足够数量的转化体。
