从戊糖片球菌中鉴定产生 D-3-苯乳酸的 D-乳酸脱氢酶
Characterization of D-lactate dehydrogenase producing D-3-phenyllactic acid from Pediococcus pentosaceus.
作者信息
Yu Shuhuai, Jiang Houyi, Jiang Bo, Mu Wanmeng
机构信息
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.
出版信息
Biosci Biotechnol Biochem. 2012;76(4):853-5. doi: 10.1271/bbb.110955. Epub 2012 Apr 7.
D-Lactate dehydrogenase (D-LDH) from Pediococcus pentosaceus ATCC 25745 was found to produce D-3-phenyllactic acid from phenylpyruvate. The optimum pH and temperature for enzyme activity were pH 5.5 and 45 °C. The Michaelis-Menten constant (K(m)), turnover number (k(cat)), and catalytic efficiency (k(cat)/K(m)) values for the substrate phenylpyruvate were estimated to be 1.73 mmol/L, 173 s(-1), and 100 (mmol/L)(-1) s(-1) respectively.
发现来自戊糖片球菌ATCC 25745的D-乳酸脱氢酶（D-LDH）可从苯丙酮酸生成D-3-苯乳酸。酶活性的最适pH和温度分别为pH 5.5和45℃。底物苯丙酮酸的米氏常数（K(m)）、转换数（k(cat)）和催化效率（k(cat)/K(m)）值分别估计为1.73 mmol/L、173 s(-1)和100 (mmol/L)(-1) s(-1)。
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