State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China.
Biotechnol Lett. 2014 Mar;36(3):627-31. doi: 10.1007/s10529-013-1404-2. Epub 2013 Nov 19.
3-Phenyllactic acid (PLA) is an antimicrobial compound with broad and effective antimicrobial activity against both bacteria and fungi. Enzymatic production of PLA can be carried out from phenylpyruvic acid by lactate dehydrogenase (LDH); however, the enzymatic reaction is accompanied by NADH oxidation that inhibits PLA biotransformation. Here, NADH regeneration was achieved using the formate dehydrogenase from Ogataea parapolymorpha and introduced into the D-PLA production process using the D-LDH from Pediococcus pentosaceus. Optimum PLA production by dual enzyme treatment was at pH 6.0 and 50 °C with both enzymes at 0.4 μM. Using 0.2 mM NADH, D-PLA production by NADH regeneration system reached 5.5 mM, which was significantly higher than that by a single-enzyme reaction.
3-苯乳酸（PLA）是一种具有广谱和高效抗菌活性的抗菌化合物，对细菌和真菌均有抑制作用。PLA 可以通过乳酸脱氢酶（LDH）从苯丙酮酸中酶法合成；然而，该酶反应伴随着 NADH 氧化，从而抑制 PLA 生物转化。在这里，使用粘红酵母中的甲酸脱氢酶进行 NADH 再生，并将其引入到戊糖片球菌的 D-LDH 中用于 D-PLA 的生产过程。双酶处理的最佳 PLA 生产条件为 pH6.0 和 50°C，两种酶的浓度均为 0.4 μM。使用 0.2 mM NADH，通过 NADH 再生系统的 D-PLA 产量达到 5.5 mM，明显高于单酶反应。
