Stochaj Wayne R, Berkelman Tom, Laird Nancy
CSH Protoc. 2006 Oct 1;2006(5):pdb.prot4543. doi: 10.1101/pdb.prot4543.
INTRODUCTIONBecause Ponceau S is relatively insensitive (~1 μg of protein), only the most abundant proteins will be visible. However, it is a reversible stain that can be removed completely with H(2)O prior to processing the blots. After staining, a soft lead pencil can be used to record the presence of visible proteins and molecular-weight markers, which will help when aligning the proteins detected on the membrane by western analysis with those in a total protein-stained gel or membrane. Ponceau S is compatible with both nitrocellulose and PVDF membranes.
引言
因为丽春红S相对不敏感（约1μg蛋白质），所以只有最丰富的蛋白质才会可见。然而，它是一种可逆染色剂，在处理印迹之前可用水完全去除。染色后，可用软铅笔记录可见蛋白质和分子量标记物的存在情况，这在将通过蛋白质印迹法在膜上检测到的蛋白质与总蛋白质染色凝胶或膜中的蛋白质进行比对时会有帮助。丽春红S与硝酸纤维素膜和聚偏二氟乙烯膜都兼容。
