Department of Neurobiology, Physiology, and Behavior, University of California, Davis, CA 95616, USA.
Anal Biochem. 2013 Sep 15;440(2):186-8. doi: 10.1016/j.ab.2013.05.027. Epub 2013 Jun 6.
Semi-quantification of proteins using Western blots typically involves normalization against housekeeping genes such as β-actin. More recently, Ponceau S and Coomassie blue staining have both been shown to be suitable alternatives to housekeeping genes as loading controls. Stain-Free total protein staining offers the advantage of no staining or destaining steps. Evaluation of the use of Stain-Free staining as an alternative to β-actin or the protein stain Ponceau S showed that Stain-Free staining was superior to β-actin and as good as or better than Ponceau S staining as a loading control for Western blots.
使用 Western blot 进行蛋白质的半定量分析通常需要针对管家基因（如β-肌动蛋白）进行归一化处理。最近，丽春红 S 和考马斯亮蓝染色均已被证明是替代管家基因作为加载对照的合适方法。无染色或脱色步骤的 Stain-Free 总蛋白染色具有优势。评估 Stain-Free 染色作为β-肌动蛋白或蛋白染色丽春红 S 的替代方法的结果表明，Stain-Free 染色优于β-肌动蛋白，并且作为 Western blot 的加载对照与丽春红 S 染色一样好或更好。
