Harlow Ed, Lane David
CSH Protoc. 2006 Nov 1;2006(6):pdb.prot4644. doi: 10.1101/pdb.prot4644.
This assay is used for protein quantitation. It is relatively accurate for most proteins, except for small basic polypeptides such as ribonuclease or lysozyme. It is also hampered by detergent concentrations over ~0.2%.
该检测方法用于蛋白质定量。对于大多数蛋白质来说，它相对准确，但对于诸如核糖核酸酶或溶菌酶等小的碱性多肽除外。洗涤剂浓度超过约0.2%时也会对其产生干扰。
