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通过胶体喷雾干燥法合成的纳米递送壳聚糖-豌豆菠萝蛋白酶水解产物（PHGPB）

Nano Delivery Chitosan-Protein/Hydrolysate of Green Peas Bromelain (PHGPB) Synthesized by Colloidal-Spray Drying Method.

作者信息

Hidayat Meilinah, Hasan Khomaini, Yusuf Muhamad, Sriwidodo Sriwidodo, Panatarani Camellia, Joni I Made

机构信息

Department of Nutrition, Faculty of Medicine, Universitas Kristen Maranatha, Jalan Suria Sumantri 65, Bandung 40164, West Java, Indonesia.

Department of Biochemistry, Faculty of Medicine, Universitas Jenderal Achmad Yani, Jalan Terusan Jenderal Sudirman, P.O. Box 148, Cimahi 40531, West Java, Indonesia.

出版信息

Polymers (Basel). 2023 May 31;15(11):2546. doi: 10.3390/polym15112546.

DOI:10.3390/polym15112546
PMID:37299345
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10255378/
Abstract

Patients with chronic kidney disease (CKD) suffer persistent decreased kidney function. Previous study of protein hydrolysate of green pea (Pisum sativum) bromelain (PHGPB) has shown promising results as an antifibrotic in glucose-induced renal mesangial culture cells, by decreasing their TGF-β levels. To be effective, protein derived from PHGPB must provide adequate protein intake and reach the target organs. This paper presents a drug delivery system for the formulation of PHGPB using chitosan as polymeric nanoparticles. A PHGPB nano delivery system was synthesized by precipitation with fixed chitosan 0.1 wt.%, followed by a spray drying process at different aerosol flow rates of 1, 3, and 5 L/min. FTIR results showed that the PHGPB was entrapped in the chitosan polymer particles. Homogeneous size and spherical morphology of NDs were obtained for the chitosan-PHGPB with a flow rate of 1 L/min. Our in vivo study showed that the highest entrapment efficiency, solubility, and sustained release were achieved by the delivery system method at 1 L/min. It was concluded that the chitosan-PHGPB delivery system developed in this study improves pharmacokinetics compared to pure PHGPB.

摘要

慢性肾脏病（CKD）患者的肾功能持续下降。先前对豌豆（Pisum sativum）菠萝蛋白酶的蛋白水解物（PHGPB）的研究表明，通过降低葡萄糖诱导的肾系膜培养细胞中的转化生长因子-β（TGF-β）水平，它作为一种抗纤维化药物具有良好的效果。为了发挥作用，源自PHGPB的蛋白质必须提供足够的蛋白质摄入量并到达靶器官。本文介绍了一种使用壳聚糖作为聚合物纳米颗粒来制备PHGPB的药物递送系统。通过用0.1 wt.%的固定壳聚糖沉淀，随后在1、3和5 L/min的不同气溶胶流速下进行喷雾干燥过程，合成了一种PHGPB纳米递送系统。傅里叶变换红外光谱（FTIR）结果表明，PHGPB被包裹在壳聚糖聚合物颗粒中。对于流速为1 L/min的壳聚糖-PHGPB，获得了均匀尺寸和球形形态的纳米颗粒（NDs）。我们的体内研究表明，通过1 L/min的递送系统方法实现了最高的包封效率、溶解度和持续释放。得出的结论是，与纯PHGPB相比，本研究中开发的壳聚糖-PHGPB递送系统改善了药代动力学。

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