大鼠肝脏环磷酸腺苷不敏感蛋白激酶对天然和部分去磷酸化酪蛋白苏氨酸和丝氨酸残基的磷酸化作用
Phosphorylation of threonine and serine residues of native and partially dephosphorylated caseins by a rat liver cyclic AMP-insensitive protein kinase.
作者信息
Deana D, Meggio F, Pinna L A
出版信息
Biochem J. 1979 Jun 1;179(3):693-6. doi: 10.1042/bj1790693.
Abstract
The preferential phosphorylation of threonine residues of native casein fractions by a rat liver cyclic AMP-independent protein kinase (EC 2.7.1.37) is abolished by preliminary limited dephosphorylation of the substrates, which promotes a fall in the phosphothreonine/phosphoserine ratios from values higher than 1 to much less than 0.1. This finding and the identification of the threonine residues phosphorylated support the view that the liver protein kinase affects threonine residues only when suitable serine residues, which fulfil the structural requirements for attack by the enzyme but which are not yet phosphorylated, are not available.
摘要
大鼠肝脏环磷酸腺苷非依赖性蛋白激酶（EC 2.7.1.37）对天然酪蛋白组分苏氨酸残基的优先磷酸化作用，会被底物的初步有限去磷酸化所消除，这会促使磷酸苏氨酸/磷酸丝氨酸的比率从高于1的值降至远低于0.1。这一发现以及对被磷酸化的苏氨酸残基的鉴定支持了这样一种观点，即肝脏蛋白激酶仅在合适的丝氨酸残基不可用时才会影响苏氨酸残基，这些丝氨酸残基满足酶攻击的结构要求但尚未被磷酸化。
相似文献
1
Phosphorylation of threonine and serine residues of native and partially dephosphorylated caseins by a rat liver cyclic AMP-insensitive protein kinase.大鼠肝脏环磷酸腺苷不敏感蛋白激酶对天然和部分去磷酸化酪蛋白苏氨酸和丝氨酸残基的磷酸化作用
Biochem J. 1979 Jun 1;179(3):693-6. doi: 10.1042/bj1790693.
2
A minor species of a type I casein kinase from yeast phosphorylating threonine residues of protein substrate.
Biochim Biophys Acta. 1985 Jan 28;838(1):171-4. doi: 10.1016/0304-4165(85)90263-6.
3
Phosphorylation of casein by mitochondrial protein kinase(s).线粒体蛋白激酶对酪蛋白的磷酸化作用
Mol Cell Biochem. 1976 Nov 30;13(2):67-71. doi: 10.1007/BF01837055.
4
Different susceptibility of whole casein components to enzymatic phosphorylation by two forms of rat liver 'casein kinase'.
Biochim Biophys Acta. 1978 Jun 9;524(2):316-26. doi: 10.1016/0005-2744(78)90168-7.
5
Sodium taurocholate cotransporting polypeptide is a serine, threonine phosphoprotein and is dephosphorylated by cyclic adenosine monophosphate.牛磺胆酸钠共转运多肽是一种丝氨酸、苏氨酸磷酸化蛋白，可被环磷酸腺苷去磷酸化。
Hepatology. 1998 Dec;28(6):1629-36. doi: 10.1002/hep.510280624.
6
Partial purification and properties of a cyclic 3',5'-AMP-independent protein kinase from rat liver.大鼠肝脏中一种不依赖环3',5'-腺苷酸的蛋白激酶的部分纯化及性质
Biochim Biophys Acta. 1977 Jan 11;480(1):122-36. doi: 10.1016/0005-2744(77)90327-8.
7
A type-1 casein kinase from yeast phosphorylates both serine and threonine residues of casein. Identification of the phosphorylation sites.来自酵母的1型酪蛋白激酶可磷酸化酪蛋白的丝氨酸和苏氨酸残基。磷酸化位点的鉴定。
Biochim Biophys Acta. 1985 Jun 10;829(2):180-7. doi: 10.1016/0167-4838(85)90187-6.
8
Dopamine- and cAMP-regulated phosphoprotein DARPP-32: phosphorylation of Ser-137 by casein kinase I inhibits dephosphorylation of Thr-34 by calcineurin.多巴胺和环磷酸腺苷调节的磷蛋白DARPP - 32：酪蛋白激酶I对丝氨酸137的磷酸化抑制了钙调磷酸酶对苏氨酸34的去磷酸化作用。
Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2682-5. doi: 10.1073/pnas.92.7.2682.
9
Characterization of serine and threonine phosphorylation sites in beta-elimination/ethanethiol addition-modified proteins by electrospray tandem mass spectrometry and database searching.通过电喷雾串联质谱法和数据库搜索对β-消除/乙硫醇加成修饰蛋白质中的丝氨酸和苏氨酸磷酸化位点进行表征。
Biochemistry. 1998 Nov 17;37(46):16211-24. doi: 10.1021/bi981264p.
10
Identification of three protein kinases which phosphorylate threonyl-tRNA synthetase from rat liver.
FEBS Lett. 1986 Oct 6;206(2):335-8. doi: 10.1016/0014-5793(86)81007-9.
本文引用的文献
1
[Preparative fractionation of caseins from cattle and sheep by chromatography on D.E.A.E. cellulose using urea and 2-mercaptoethanol].[使用尿素和2-巯基乙醇通过DEAE-纤维素柱色谱法对牛和羊的酪蛋白进行制备性分级分离]
Bull Soc Chim Biol (Paris). 1968;50(3):521-30.
2
Phosphoprotein kinases from rat liver cytosol.来自大鼠肝脏胞质溶胶的磷蛋白激酶。
Biochim Biophys Acta. 1972 Nov 10;289(1):237-41. doi: 10.1016/0005-2744(72)90127-1.
3
The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters.直接线性作图法。一种用于估算酶动力学参数的新的图形方法。
Biochem J. 1974 Jun;139(3):715-20. doi: 10.1042/bj1390715.
4
[Primary structure of bovine kappa B casein. Complete sequence].[牛κ-酪蛋白的一级结构。完整序列]
Eur J Biochem. 1973 Jun;35(2):222-35. doi: 10.1111/j.1432-1033.1973.tb02829.x.
5
[Primary structure of bovine beta casein. Complete sequence].[牛β-酪蛋白的一级结构。完整序列]
Eur J Biochem. 1972 Feb;25(3):505-14. doi: 10.1111/j.1432-1033.1972.tb01722.x.
6
[Primary structure of bovine s1 casein. Complete sequence].[牛s1酪蛋白的一级结构。完整序列]
Eur J Biochem. 1971 Nov 11;23(1):41-51. doi: 10.1111/j.1432-1033.1971.tb01590.x.
7
Comparative study of mitochondrial and cytosol protein kinase activities.线粒体和胞质溶胶蛋白激酶活性的比较研究
Biochim Biophys Acta. 1976 Dec 21;451(2):484-90. doi: 10.1016/0304-4165(76)90143-4.
8
[The primary structure of bovine alpha s2 caseins].[牛αs2酪蛋白的一级结构]
FEBS Lett. 1976 Nov 15;72(1):111-6. doi: 10.1016/0014-5793(76)80910-6.
9
Decomposition of phosphoserine and phosphothreonine during acid hydrolysis.酸性水解过程中磷酸丝氨酸和磷酸苏氨酸的分解
Anal Biochem. 1976 Jun;73(2):477-85. doi: 10.1016/0003-2697(76)90197-4.
10
Specific casein phosphorylation by a casein kinase from lactating bovine mammary gland.来自泌乳期奶牛乳腺的酪蛋白激酶对特定酪蛋白的磷酸化作用。
Eur J Biochem. 1977 Jun 1;76(1):233-43. doi: 10.1111/j.1432-1033.1977.tb11588.x.
Zotero 插件