Bhandal Jitender, Langohr Ingeborg M, Degner Daniel A, Xie Yan, Stanley Bryden J, Walshaw Richard
Michigan Veterinary Specialists, Department of Surgery, Southfield, MI, USA.
Vet Surg. 2012 May;41(4):448-54. doi: 10.1111/j.1532-950X.2012.00987.x. Epub 2012 Apr 6.
To (1) compare thickness of the epidermis and dermis of anatomically different donor sites in dogs, and (2) evaluate hair follicle damage during full thickness skin grafts (FTSG) preparation from anatomically different donor sites. Another objective was to compare the extent of agreement between gross and histologic quality of graft preparation.
Ex vivo study.
Healthy Beagle dogs (n = 12).
Skin samples were harvested from fresh cadavers free of gross dermatopathology and FTSG prepared. Regional, epidermal and dermal thickness, and hair follicle density in intact skin and FTSG specimens from different regions were determined by histomorphometric analysis. Hair follicle density in intact skin and FTSG specimens, and skin thickness measurements were compared among regions.
Mean epidermis and dermis thickness of the dorsal lumbar and lateral neck regions were significantly greater when compared with other sites. Total hair follicle (superficial and deep dermis) densities were significantly less in prepared FTSG versus intact skin specimens. The dorsal lumbar region had the highest hair follicle density.
Thickness of the epidermis and dermis is dependent on body region. Hair follicle density in the FTSG specimens was decreased compared with intact skin specimens, which may affect hair regrowth in FTSG.
（1）比较犬类不同解剖供区的表皮和真皮厚度，（2）评估从不同解剖供区制备全层皮肤移植（FTSG）时毛囊的损伤情况。另一个目的是比较移植制备的大体质量和组织学质量之间的一致性程度。
体外研究。
健康比格犬（n = 12）。
从无明显皮肤病理学病变的新鲜尸体上采集皮肤样本并制备FTSG。通过组织形态计量分析确定不同区域完整皮肤和FTSG标本的区域、表皮和真皮厚度以及毛囊密度。比较不同区域完整皮肤和FTSG标本的毛囊密度以及皮肤厚度测量值。
与其他部位相比，背腰部和侧颈部区域的平均表皮和真皮厚度明显更大。制备的FTSG标本中总的毛囊（浅表和深部真皮）密度明显低于完整皮肤标本。背腰部区域的毛囊密度最高。
表皮和真皮的厚度取决于身体部位。与完整皮肤标本相比，FTSG标本中的毛囊密度降低，这可能会影响FTSG中的毛发再生。
