Department of Pathology, Forensic Sciences, University Hospital and School of Medicine of Santiago de Compostela, Servicio de Anatomia Patológica, Hospital Clinico de Santiago, Santiago de Compostela, Spain.
J Oral Pathol Med. 2012 Oct;41(9):667-74. doi: 10.1111/j.1600-0714.2012.01147.x. Epub 2012 Apr 4.
Carbonic anhydrases (CAs), a group of ubiquitously expressed metalloenzymes, are involved in numerous physiological and pathological processes, including tumorigenicity. Specifically, CA-IX has been primarily found in hypoxic tumor tissues.
This is a retrospective study of tumors from the Tissue Bank of the Pathology Department of the University Hospital of Santiago de Compostela. We selected 50 oral squamous cell carcinomas (OSCCs) using Tissue Microarray (TMA) technology. The immunohistochemical study was performed to determine CA-IX expression. The resulting data were subject to statistical analysis and survival curves.
Of the 50 cases, 23 were detected in early stages (I and II) and 27 in advanced stages (III and IV). In the first year, almost 50% of patients in stages III-IV died, which contrasted with those patients in initial stages who registered a survival rate of 80% (P = 0.019). Regarding the expression of CA-IX, nine cases (18%) were negative, 18 cases (36%) were moderate, while 23 cases (46%) were intense. Tumors in stages I-II showed a positivity of 52.6%; however, in advanced stages, the percentage reached 95.5% (P = 0.002). Regarding CA-IX expression and survival, patients with tumors with strong staining had a lower average survival time (13.8 months) than patients with negative or weak-moderate staining (33.4 and 32.8 months, respectively), log-rank=6.1, P value=0.0484.
Early diagnosis of these tumors is essential to improve patient survival. CA-IX expression augments with increasing tumor stage, probably related with the degree of hypoxia; thus, its measurement can be used as a prognostic factor.
碳酸酐酶（CA）是一组广泛表达的金属酶，参与许多生理和病理过程，包括肿瘤发生。具体来说，CA-IX 主要存在于缺氧的肿瘤组织中。
这是对圣地亚哥德孔波斯特拉大学医院病理科组织库肿瘤的回顾性研究。我们使用组织微阵列（TMA）技术选择了 50 例口腔鳞状细胞癌（OSCC）。进行免疫组织化学研究以确定 CA-IX 的表达。对所得数据进行统计分析和生存曲线分析。
在 50 例病例中，有 23 例被检测为早期（I 和 II 期），27 例为晚期（III 和 IV 期）。在第一年，III-IV 期的近 50%的患者死亡，而处于初始阶段的患者的生存率为 80%（P=0.019）。关于 CA-IX 的表达，有 9 例（18%）为阴性，18 例（36%）为中度，23 例（46%）为强阳性。I-II 期肿瘤的阳性率为 52.6%；然而，在晚期，该百分比达到 95.5%（P=0.002）。关于 CA-IX 表达与生存，强染色肿瘤患者的平均生存时间（13.8 个月）低于阴性或弱中度染色肿瘤患者（分别为 33.4 和 32.8 个月），对数秩检验=6.1，P 值=0.0484。
早期诊断这些肿瘤对于提高患者的生存率至关重要。CA-IX 的表达随着肿瘤分期的增加而增加，可能与缺氧程度有关；因此，其测量可作为预后因素。
