Department of Microbiology, Oregon State University, Corvallis, OR 97331, USA.
J Eukaryot Microbiol. 2012 May-Jun;59(3):258-67. doi: 10.1111/j.1550-7408.2012.00619.x. Epub 2012 Apr 9.
Gonadal infections by a novel microsporidium were discovered in 34% (13/38) of arrow gobies, Clevelandia ios, sampled over a 3-yr period from Morro Bay Marina in Morro Bay, California. Gonadal tumors had been reported in arrow gobies from this geographic area. The infected gonads, found primarily in females, typically appeared grossly as large, white-gray firm and lobulated masses. Histological examination revealed large, multilobate xenomas within the ovaries and no evidence of neoplasia. Typical of the genus Ichthyosporidium, the large xenomas were filled with developmental stages and pleomorphic spores. Wet mount preparations showed two general spore types: microspores with mean length of 6.2 (7.0-4.9, SD = 0.6, N = 20) μm and mean width of 4.3 (5.3-2.9, SD = 0.8) μm; and less numerous macrospores with mean length of 8.5 (10.1-7.1, SD = 1.0, N = 10) μm and mean width of 5.5 (6.2-4.8, SD = 0.5) μm. Transmission electron microscopy demonstrated stages consistent with the genus and 35-50 turns of the polar filament. Small subunit rDNA gene sequence analysis revealed that the parasite from arrow gobies was most closely related to, but distinct from Ichthyosporidium sp. based on sequences available in GenBank. We conclude that this microsporidium represents a new species of Ichthyosporidium, the first species of this genus described from a member of the family Gobiidae and from the Pacific Ocean.
在加利福尼亚州莫罗湾莫罗湾码头采集的 3 年期间，34%（13/38）的 Arrow Gobies，Clevelandia ios 中发现了一种新型微孢子虫的生殖器官感染。来自该地理区域的 Arrow Gobies 已报告有生殖器官肿瘤。受感染的生殖器官主要在雌性中发现，通常表现为大的、灰白色的、坚硬的、分叶状的肿块。组织学检查显示卵巢内有大的、多叶的异种瘤，没有肿瘤的证据。典型的 Ichthyosporidium 属，大的异种瘤充满了发育阶段和多形孢子。湿装片制备显示两种一般的孢子类型：微孢子，平均长度为 6.2（7.0-4.9，SD=0.6，N=20）μm，平均宽度为 4.3（5.3-2.9，SD=0.8）μm；数量较少的大孢子，平均长度为 8.5（10.1-7.1，SD=1.0，N=10）μm，平均宽度为 5.5（6.2-4.8，SD=0.5）μm。透射电子显微镜显示与属一致的阶段和极丝的 35-50 圈。小亚单位 rDNA 基因序列分析表明，来自 Arrow Gobies 的寄生虫与 Ichthyosporidium sp. 最为密切相关，但与基于 GenBank 中可用序列的 Ichthyosporidium sp. 不同。我们得出结论，这种微孢子虫代表了 Ichthyosporidium 的一个新种，这是该属的第一个从 Gobidae 科的成员和太平洋描述的物种。
