Ovcharenko Mykola, Wróblewski Piotr, Kvach Yuriy, Drobiniak Oleksandra
Institute of Biology and Environmental Protection, Pomeranian University, 22b Arciszewskiego, 76-200, Słupsk, Poland.
Witold Stefański Institute of Parasitology of the Polish Academy of Sciences, 51/55 Twarda Street, 00-818, Warsaw, Poland.
Parasitol Res. 2017 May;116(5):1453-1462. doi: 10.1007/s00436-017-5422-1. Epub 2017 Mar 23.
A previously recognized Glugea-belonging Microsporidia (G. melanostomi, G. markievichi, G. naidenovi) were found in the intestinal mucosa of Neogobius melanostomus, N. fluviatilis, and Proterorhinchus semilunaris (Teleostei, Gobiidae) from lower Dnieper River and Black Sea coastal inlets, Ukraine. Light microscope observations demonstrated small spherical xenomes about 0.35 mm in diameter filled by developmental stages of parasites. Mature spores were ellipsoidal, slightly narrowed at anterior pole, sometimes ovoid or elongate in shape approximately 3.1-4.5 × 2.0-2.9 μm in size. The stained spores were elongate ovoid that are measured about 2.3-4.3 × 1.6-3.4 μm. Ultrastructural study showed that xenoma wall consisted of finely granular layer up to 350 nm wide. Numerous host-derived collagenous fibers adhered to the xenoma wall were noticed. Microtubular inclusions about 40 nm in diameter occupied the episporontal space. Mature spores displayed the polar filament arranged in one row. One to two last turns of polar filament were sharply tapered to half of diameter. The number of polar filament turns varied depending of host infected from 10 (9 + 1) in N. melanostomus to 13 (11 + 2) in N. fluviatilis and 14 (13 + 1) in P. semilunaris. The endospore thickness varied depending of host infected from 30-35 nm in case of N. fluviatiis to 40-50 nm in P. semilunaris and 60-80 nm in N. melanostomus. SSU 16S rRNA sequence analysis, blast searches, and ultrastuctural data revealed similarity of all studied microsporidians with Loma acerinae (Jirovec, 1930).
在乌克兰第聂伯河下游和黑海沿岸海湾的黑口新鰕虎鱼、河川新鰕虎鱼和半月亮前肛鳗（硬骨鱼纲，鰕虎鱼科）的肠黏膜中发现了先前已确认的属于格鲁吉亚属的微孢子虫（黑口格鲁吉亚微孢子虫、马尔基耶维奇格鲁吉亚微孢子虫、纳伊德诺维格鲁吉亚微孢子虫）。光学显微镜观察显示，寄生虫发育阶段充满了直径约0.35毫米的小球形异染粒。成熟孢子呈椭圆形，在前极处略有变窄，有时呈卵形或细长形，大小约为3.1 - 4.5×2.0 - 2.9微米。染色后的孢子呈细长卵形，测量值约为2.3 - 4.3×1.6 - 3.4微米。超微结构研究表明，异染粒壁由宽度达350纳米的细颗粒层组成。注意到许多源自宿主的胶原纤维附着在异染粒壁上。直径约40纳米的微管内含物占据了孢子周质空间。成熟孢子的极丝排成一排。极丝的最后一到两圈急剧变细至直径的一半。极丝的圈数因感染的宿主而异，在黑口新鰕虎鱼中为10（9 + 1）圈，在河川新鰕虎鱼中为13（11 + 2）圈，在半月亮前肛鳗中为14（13 + 1）圈。内膜厚度因感染的宿主而异，在河川新鰕虎鱼中为30 - 35纳米，在半月亮前肛鳗中为40 - 50纳米，在黑口新鰕虎鱼中为60 - 80纳米。小亚基16S rRNA序列分析、比对搜索和超微结构数据显示，所有研究的微孢子虫与acerinae洛马微孢子虫（伊罗韦茨，1930年）具有相似性。
