Pang Yu, Li Guo-jian, Wu Ji-zhou, Wu Jian-lin, Chen Wu-qing, Ning Qiu-yue, Wei Ying-hua, Hu Die-fei, Qin Ling
Department of Infectious Diseases, Guangxi Medical University, Nanning, Guangxi, People's Republic of China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2012 Apr;29(2):206-9. doi: 10.3760/cma.j.issn.1003-9406.2012.02.019.
To assess the correlation between familial clustering of hepatocellular carcinoma (HCC) and the level of anti-P53 in human serum in Guangxi.
Enzyme-linked immunosorbent assay (ELISA) was used to detect anti-P53 in 164 members from 20 HCC families and 164 members from non-cancer control families. Univariate analysis was performed to assess the correlation between seral level of P53 antibody and familial clustering of HCC.
The level of P53 antibody was significantly higher in the members of HCC families than controls (Z=-3.04, P=0.002). After eliminating the interference of hepatitis B virus infection, this tendency still remains (P=0.011). And there was a significant difference between relatives of different degrees from HCC families (chi-square=11.593, P=0.021), with the expression of anti-P53 declining along with decrease in relationship coefficient. Furthermore, the number of individuals with high anti-P53 expression was also significantly greater in HCC families (95/164) than controls (71/164) (P=0.006). And the expression was rising along with the increasing HCC numbers (chi-square=16.068, P=0.000). Anti-P53 level was also greater in HCC families featuring sibling affection than parental affection (chi-square=12.679, P=0.002). Univariate analysis indicated that high expression of anti-P53 is a risk factor for development of HCC (OR=2.087, 95%CI: 1.270-3.431).
High level of anti-P53 expression may be a factor for the clustering of HCC families in Guangxi, China.
评估广西地区肝细胞癌（HCC）家族聚集性与人体血清中抗P53水平之间的相关性。
采用酶联免疫吸附测定（ELISA）法检测20个HCC家族的164名成员及非癌对照家族的164名成员血清中的抗P53。进行单因素分析以评估P53抗体血清水平与HCC家族聚集性之间的相关性。
HCC家族成员的P53抗体水平显著高于对照组（Z = -3.04，P = 0.002）。排除乙型肝炎病毒感染的干扰后，这种趋势仍然存在（P = 0.011）。HCC家族不同程度亲属之间存在显著差异（χ² = 11.593，P = 0.021），抗P53的表达随亲缘系数降低而下降。此外，抗P53高表达个体的数量在HCC家族（95/164）中也显著多于对照组（71/164）（P = 0.006）。并且其表达随HCC病例数增加而上升（χ² = 16.068，P = 0.000）。在以兄弟姐妹关系为主的HCC家族中抗P53水平也高于以父母子女关系为主的家族（χ² = 12.679，P = 0.002）。单因素分析表明抗P53高表达是HCC发生的危险因素（OR = 2.087，95%CI：1.270 - 3.431）。
抗P53高表达可能是中国广西地区HCC家族聚集的一个因素。
