Celik Mustafa S, Gur Ali, Akdağ Zulkuf, Akpolat Veysi, Guven Kemal, Celik Yusuf, Sarac Aysegul J, Otçu Selcuk
Department of Biophysics, Medical Faculty of Dicle University, Diyarbakir, Turkey.
Bioelectromagnetics. 2012 Oct;33(7):543-9. doi: 10.1002/bem.21725. Epub 2012 Apr 9.
The effects of long-term extremely low-frequency magnetic field (ELF-MF) exposure on bone formation and biochemical markers were investigated in ovariectomized rats. Sixty mature female Sprague-Dawley rats were randomly divided into four different groups (n = 15): (i) unexposed control (CTL); (ii) ovariectomized only (OVX); (iii) non-ovariectomized, exposed (SHAM + ELF-MF); and (iv) ovariectomized, exposed (OVX + ELF-MF). The third and fourth groups were exposed to 1.5 mT ELF-MF for 4 h a day for 6 months. Bone mineral density (BMD) was determined using dual energy X-ray absorption (DEXA) measurements. The formation and resorption of bone were evaluated using bone-specific alkaline phosphatase (BAP), osteocalcin, osteoprotogerin, and N-telopeptide. After 6 months of ELF-MF therapy, BMD values were significantly lower in the OVX group and higher in the OVX + ELF-MF and SHAM + ELF-MF groups than they were before therapy (P < 0.001). Although there was no significant difference in BMD values among the groups before therapy, the BMD values increased significantly after 6 months in the OVX + ELF-MF and SHAM + ELF-MF groups and were reduced in the OVX group compared to the CTL group (P < 0.001). The concentrations of BAP, osteocalcin, osteoprotogerin, and N-telopeptide in the three experimental groups also changed in a significant way compared to the CTL group. The results of the present study suggest that osteoporosis can be inhibited by ELF-MF stimulation treatments. It was also concluded that ELF-MF may be useful in the prevention of osteoporosis in ovariectomized rats.
研究了长期暴露于极低频磁场（ELF-MF）对去卵巢大鼠骨形成和生化标志物的影响。60只成熟雌性Sprague-Dawley大鼠随机分为四组（n = 15）：（i）未暴露对照组（CTL）；（ii）仅去卵巢组（OVX）；（iii）未去卵巢且暴露组（SHAM + ELF-MF）；（iv）去卵巢且暴露组（OVX + ELF-MF）。第三组和第四组每天暴露于1.5 mT的ELF-MF中4小时，持续6个月。使用双能X线吸收法（DEXA）测量骨密度（BMD）。通过骨特异性碱性磷酸酶（BAP）、骨钙素、骨保护素和N-端肽评估骨的形成和吸收。ELF-MF治疗6个月后，OVX组的BMD值显著低于治疗前，而OVX + ELF-MF组和SHAM + ELF-MF组的BMD值高于治疗前（P < 0.001）。虽然治疗前各组的BMD值无显著差异，但6个月后，OVX + ELF-MF组和SHAM + ELF-MF组的BMD值显著升高，与CTL组相比，OVX组的BMD值降低（P < 0.001）。与CTL组相比，三个实验组中BAP、骨钙素、骨保护素和N-端肽的浓度也发生了显著变化。本研究结果表明，ELF-MF刺激治疗可抑制骨质疏松症。还得出结论，ELF-MF可能有助于预防去卵巢大鼠的骨质疏松症。
