Kiss C, Balázs M, Kovács I, Karmazsin L
Department of Paediatrics, University Medical School Debrecen, Hungary.
Acta Paediatr Hung. 1990;30(2):291-302.
Plasma membrane fluidity has been investigated by determining steady-state fluorescence polarization (FP) of the apolar stain 1,6-diphenyl-1,3,5,-hexatriene in intact blast cells, separated from peripheral blood and bone marrow of children with various types of acute leukaemia. FP-values of blast cells taken before antileukaemic therapy were compared with FP-values of peripheral blood and bone marrow mononuclear cells separated from patients in complete remission as well as from control patients and from healthy volunteers. Moreover, fluorescence polarization measurements were also performed using blast cells of leukaemic patients on short-term single-drug prednisolone pretreatment. The results have shown that untreated blast cells have significantly lower FP-values than normal mononuclear cells of peripheral blood or bone marrow. No compartment difference has been observed within blast cells, while normal mononuclear cells from peripheral blood have significantly higher FP-values than bone marrow cells. FP-values of cells separated in remission or during prednisolone treatment do not differ from control values.
通过测定非极性染料1,6 - 二苯基 - 1,3,5 - 己三烯在完整原始细胞中的稳态荧光偏振（FP），对来自患有各种类型急性白血病儿童的外周血和骨髓中分离出的原始细胞膜流动性进行了研究。将抗白血病治疗前采集的原始细胞的FP值与完全缓解患者、对照患者以及健康志愿者外周血和骨髓单个核细胞的FP值进行比较。此外，还使用白血病患者的原始细胞在短期单药泼尼松龙预处理后进行了荧光偏振测量。结果表明，未经治疗的原始细胞的FP值显著低于外周血或骨髓的正常单个核细胞。原始细胞内未观察到区室差异，而外周血的正常单个核细胞的FP值显著高于骨髓细胞。缓解期或泼尼松龙治疗期间分离的细胞的FP值与对照值无差异。
