Shanghai Key Laboratory of Functional Materials Chemistry, and Research Centre of Analysis and Test, East China Univ. of Science and Technology, Meilong Rd 130, Shanghai 200237, China.
J Food Sci. 2012 May;77(5):T105-9. doi: 10.1111/j.1750-3841.2012.02665.x. Epub 2012 Apr 10.
An ultrasensitive method based on Surface enhanced Raman scattering (SERS) has been developed to determine content of a pesticide which is tricyclazole in paddy rice using sliver colloid as a substrate and pyridine as an internal standard. The peaks at 424 and 1035 cm(-1) in a SERS spectrum were selected as analytic and internal peaks, respectively, and their intensity ratio I(t)/I(p) was used to calculate the regression concentration of tricyclazole. The correlation between I(t)/I(p) and concentration showed significant linear relationship with a correlation coefficient of R(2)= 0.995 in a concentration range of 0.05 to 0.70 mg/L and the tricyclazole solution can be detected to be low as 0.002 mg/L by SERS. The method was applied to determine tricyclazole contents of 3 real rice samples with a standard addition method in order to eliminate interference of matrix. The errors of SERS measurements for the 3 samples were 0.0008 to 0.0246, 0.0013 to 0.0028, and 0.0129 to 0.0304 mg/kg, respectively, compared with the results obtained by high performance liquid chromatography method. This also showed a good reproducibility with low values of relative standard deviation (n= 3) for the 3 samples ranged from 3.63% to 4.64%.
一种基于表面增强拉曼散射（SERS）的高灵敏度方法已被开发出来，用于通过使用银胶体作为基底和吡啶作为内标来测定稻谷中三唑酮农药的含量。在 SERS 光谱中选择 424 和 1035 cm(-1) 处的峰分别作为分析峰和内标峰，并用它们的强度比 I(t)/I(p) 来计算三唑酮的回归浓度。I(t)/I(p)与浓度之间的相关性呈现出显著的线性关系，在 0.05 至 0.70 mg/L 的浓度范围内，相关系数 R(2)=0.995，并且可以通过 SERS 检测到浓度低至 0.002 mg/L 的三唑酮溶液。该方法被应用于用标准添加法测定 3 个实际稻米样品中的三唑酮含量，以消除基质的干扰。与高效液相色谱法的结果相比，SERS 测量这 3 个样品的误差分别为 0.0008 至 0.0246、0.0013 至 0.0028 和 0.0129 至 0.0304 mg/kg。这也表现出良好的重现性，3 个样品的相对标准偏差（n=3）值较低，范围为 3.63%至 4.64%。
