Belokoneva O S, Kolomiĭtseva G Ia
Nauchnye Doki Vyss Shkoly Biol Nauki. 1990(7):157-60.
The scintillation toluene-fenoxol 8/10 system with ammonia (0.01%) is suggested for radioactive counting in radioreceptor biomembrane assay. It is shown that chemiluminescence in this system is defined mainly by membrane components presence. The remotion of filter from scintillator after labelled molecules elution and methanol (3%) addition to scintillation mixture are suggested to reduce the chemiluminescence level.
建议在放射受体生物膜分析中使用含氨(0.01%)的闪烁甲苯 - 酚恶醚8/10系统进行放射性计数。结果表明,该系统中的化学发光主要由膜成分的存在决定。建议在标记分子洗脱后从闪烁体中移除滤器,并向闪烁混合物中加入甲醇(3%)以降低化学发光水平。