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正常人吞噬细胞中的化学发光反应。I. 使用标准液体闪烁计数器的自动测量

Chemiluminescence response in normal human phagocytes. I. Automated measurements using a standard liquid scintillation counter.

作者信息

Robinson J P, Penny R

出版信息

J Clin Lab Immunol. 1982 Apr;7(3):215-7.

PMID:7097747
Abstract

We present a fast, simple and highly accurate method for measuring Phagocyte Chemiluminescence by using a standard liquid scintillation counter with a simple modification. The method allows the measurement of multiple samples automatically, with standardized mixing and measurement times, at a constant temperature. This is achieved with a minimum of technician time as opposed to the single measurement facility of many of the available manual luminescence photometers. The modifications can be made to any standard liquid scintillation counter, are simple and inexpensive, can allow the handling of large numbers of samples in a short time and improve standardization.

摘要

我们提出了一种快速、简单且高度准确的方法,通过对标准液体闪烁计数器进行简单改装来测量吞噬细胞化学发光。该方法能够在恒定温度下,以标准化的混合和测量时间自动测量多个样本。与许多现有的手动发光光度计的单次测量功能相比,此方法只需最少的技术人员时间。这些改装可以应用于任何标准液体闪烁计数器,操作简单且成本低廉,能够在短时间内处理大量样本并提高标准化程度。

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